Your first step is to look at your images and see what is going on in that shell. Since you are looking at merged stats the first guess is that there is something wrong with all of the images, but only by looking at them can you tell.
Dale Tronrud On 8/15/2017 9:39 AM, rohit kumar wrote: > Dear All, > > > > I am refining a data in refmac5 and the data resolution is 1.8 A. Data > is looking fine with the data statics see below for data statics > > > > Inline image 1 > > > > Right now the R/Rfree is 22/26 not good for such resolution. I have > tried most of the options in refmec5 but still I am not able to lower > these R/Rfree values. > > During refinement with phenix what I found that at resolution 1.93 to > 1.86 R/Rfree is quite high see below > > > Inline image 2 > > > > > Is this the possible reason for high R/Rfree value?. If it is please > let me know how some can remove these frames during refinement. or > > > Please tell me other strategies to lower down the R/Rfree values. > > > Thank you in advance > > > With Regards > > Dr. Rohit Kumar Singh > >
