> On Feb 7, 2017, at 11:58 AM, Jonathan Grimes <jonat...@strubi.ox.ac.uk> wrote: > > > dear all, > > is there a way of refining dsRNA into low res maps (6-8A) …obvious major > and minor grooves….
With good phases, yes ? I've been able to trace multiway junctions at 5-6A. > where the base pairs are restrained but the dsRNA can curve…. > Can curve ? A little ambiguous. An kink turn can drastically change the direction of a helix. A bulge less so. A GU wobble in the middle of a helix much less so. If you have good phases at this resolution (clear phosphates) you might find RCRaNe helpful. F
