Re: [Felvtalk] Doxycycline
Amani - this is very interesting, at least the part that I understood :) I agree that Big Pharma seems to just follow the money, it's not about helping our fellow man (or kitty) anymore, sadly. And banning certain medicines because of their stigma, or potential use is just silly!!! Ardy -Original Message- From: Felvtalk [mailto:felvtalk-boun...@felineleukemia.org] On Behalf Of Amani Oakley Sent: Sunday, June 25, 2017 6:56 AM To: felvtalk@felineleukemia.org Subject: Re: [Felvtalk] Doxycycline Ardy - there are a number of publications which deal with the mode of action of Doxycycline and its antiviral properties. It doesn’t kill the virus but inhibits its replication, sometimes by interfering with cell wall development and other times by preventing the virus from penetrating cells. Viruses usually replicate by invading a normal host cell, taking over its "machinery" and basically getting the host cell to make millions more viruses, which are then released into the circulation when the cell bursts. Doxycycline has been shown to sometimes inhibit the penetration of the virus into the cell. So for example, Doxycycline has been found to be effective against Dengue fever. There are a number of publications exploring the effectiveness with Dengue fever. Here is an excerpt from a 2014 publication from the Archives of Virology (Inhibitory Effect of Doxycycline against Dengue Virus Replication in Vitro): "Doxycycline is an antibiotic derived from tetracycline that possesses antimicrobial and anti-inflammatory activities. Antiviral activity of doxycycline against dengue virus has been reported previously; however, its anti-dengue properties need further investigation. This study was conducted to determine the potential activity of doxycycline against dengue virus replication in vitro. Doxycycline inhibited the dengue virus serine protease (DENV2 NS2B-NS3pro) with an IC50 value of 52.3 ± 6.2 μM at 37 °C (normal human temperature) and 26.7 ± 5.3 μM at 40 °C (high fever temperature). The antiviral activity of doxycycline was first tested at different concentrations against DENV2 using a plaque-formation assay. The virus titter decreased significantly after applying doxycycline at levels lower than its 50 % cytotoxic concentration (CC50, 100 μM), showing concentration-dependent inhibition with a 50 % effective concentration (EC50) of approximately 50 μM. Doxycycline significantly inhibited viral entry and post-infection replication of the four dengue serotypes, with serotype-specific inhibition (high activity against DENV2 and DENV4 compared to DENV1 and DENV3). Collectively, these findings underline the need for further experimental and clinical studies on doxycycline, utilizing its anti-dengue and anti-inflammatory activities to attenuate the clinical symptoms of dengue virus infection." Doxycycline has also been found to be effective against West Nile virus, reovirus, Japanese encephalitis virus (JEV), vesicular stomatitis virus (in vitro), mural virus (a mouse virus which causes leukemia in mice), and more. Doxycycline is also effective against more unusual organisms like protozoa (eg - malaria) and the unusual bacteria which cause Lyme Disease and Rocky Mountain Spotted Fever. Unbelievably, Doxycycline has also been shown to suppress metastatic cancer or assist in bone metastatses. I suspect this last finding is not a strong one since I don’t know that Doxycycline is routinely used for this. However, I sure wish I had known this when my family member was suffering from cancer. Again, under the category of "it might not do a darn thing but it's unlikely to be worse than the situation you're facing", I would have given this a try. There are quite a few publications looking at the use of Doxy for cancer though, so I think there is probably something there. I doubt that anyone is studying the effect on the Feline Leukemia virus because I see little research involving cat conditions generally, and even less focused on the Feline Leukemia virus. I obviously went looking for an explanation as to why Doxycycline and Winstrol might have been effective with Zander, because I too had the general understanding that antibiotics are not effective against viruses (and I have a Microbiology degree!). I suspect that most physicians and vets have also had this "rule of thumb" hammered into their heads. It is generally true of course, but it obviously works to create a blind spot for physicians and vets, when it comes to the "exceptions" to this general rule. Also, what I have also come to understand is that when there is a drug like Doxycycline (and Winstrol), which has been around for a long time and the drug companies no longer have a patent on it, they can't make money on it, so they stop doing any research related to it. They focus their research on their new drugs on which they hold patents, so they can pu
Re: [Felvtalk] Doxycycline
Ardy - there are a number of publications which deal with the mode of action of Doxycycline and its antiviral properties. It doesn’t kill the virus but inhibits its replication, sometimes by interfering with cell wall development and other times by preventing the virus from penetrating cells. Viruses usually replicate by invading a normal host cell, taking over its "machinery" and basically getting the host cell to make millions more viruses, which are then released into the circulation when the cell bursts. Doxycycline has been shown to sometimes inhibit the penetration of the virus into the cell. So for example, Doxycycline has been found to be effective against Dengue fever. There are a number of publications exploring the effectiveness with Dengue fever. Here is an excerpt from a 2014 publication from the Archives of Virology (Inhibitory Effect of Doxycycline against Dengue Virus Replication in Vitro): "Doxycycline is an antibiotic derived from tetracycline that possesses antimicrobial and anti-inflammatory activities. Antiviral activity of doxycycline against dengue virus has been reported previously; however, its anti-dengue properties need further investigation. This study was conducted to determine the potential activity of doxycycline against dengue virus replication in vitro. Doxycycline inhibited the dengue virus serine protease (DENV2 NS2B-NS3pro) with an IC50 value of 52.3 ± 6.2 μM at 37 °C (normal human temperature) and 26.7 ± 5.3 μM at 40 °C (high fever temperature). The antiviral activity of doxycycline was first tested at different concentrations against DENV2 using a plaque-formation assay. The virus titter decreased significantly after applying doxycycline at levels lower than its 50 % cytotoxic concentration (CC50, 100 μM), showing concentration-dependent inhibition with a 50 % effective concentration (EC50) of approximately 50 μM. Doxycycline significantly inhibited viral entry and post-infection replication of the four dengue serotypes, with serotype-specific inhibition (high activity against DENV2 and DENV4 compared to DENV1 and DENV3). Collectively, these findings underline the need for further experimental and clinical studies on doxycycline, utilizing its anti-dengue and anti-inflammatory activities to attenuate the clinical symptoms of dengue virus infection." Doxycycline has also been found to be effective against West Nile virus, reovirus, Japanese encephalitis virus (JEV), vesicular stomatitis virus (in vitro), mural virus (a mouse virus which causes leukemia in mice), and more. Doxycycline is also effective against more unusual organisms like protozoa (eg - malaria) and the unusual bacteria which cause Lyme Disease and Rocky Mountain Spotted Fever. Unbelievably, Doxycycline has also been shown to suppress metastatic cancer or assist in bone metastatses. I suspect this last finding is not a strong one since I don’t know that Doxycycline is routinely used for this. However, I sure wish I had known this when my family member was suffering from cancer. Again, under the category of "it might not do a darn thing but it's unlikely to be worse than the situation you're facing", I would have given this a try. There are quite a few publications looking at the use of Doxy for cancer though, so I think there is probably something there. I doubt that anyone is studying the effect on the Feline Leukemia virus because I see little research involving cat conditions generally, and even less focused on the Feline Leukemia virus. I obviously went looking for an explanation as to why Doxycycline and Winstrol might have been effective with Zander, because I too had the general understanding that antibiotics are not effective against viruses (and I have a Microbiology degree!). I suspect that most physicians and vets have also had this "rule of thumb" hammered into their heads. It is generally true of course, but it obviously works to create a blind spot for physicians and vets, when it comes to the "exceptions" to this general rule. Also, what I have also come to understand is that when there is a drug like Doxycycline (and Winstrol), which has been around for a long time and the drug companies no longer have a patent on it, they can't make money on it, so they stop doing any research related to it. They focus their research on their new drugs on which they hold patents, so they can push these, and make more money. And don’t even get me started on cannabis oil. To imagine where society might have been if we'd focused on researching the effects of cannabis on everything from cold sores to cancer for the last 100 years, is to truly despair at the sheer stupidity and duplicity of governments and certain people with devious designs. Oh, and cannabis' entirely benign cousin - hemp - which got swept into the ban for no legitimate reason at all - is able to be used for everything from a food source, to clothing, to fertilizer, to
