Subodh,

I'm ccing the Apollo list back into the thread, just for future reference.

Dave C

On Wed, Dec 30, 2009 at 11:23 AM, Scott Cain <[email protected]> wrote:
> Hi Subodh,
>
> I suggest you look at MAKER for doing the prediction: it will handle
> most of your work for you (once you have it installed).
>
>  http://www.yandell-lab.org/software/maker.html
>
> Scott
>
>
> On Wed, Dec 30, 2009 at 2:20 PM, subodh srivastava
> <[email protected]> wrote:
>>
>> Thank you very much for the mail and information.
>>
>> Can you please explain little more about GFF3 files of  organism's genome 
>> annotation,
>> like, how to creat and example of any small GFF3 files.
>>
>> I want to use Fgenesh for gene prediction
>>
>> so the steps will be :
>>
>> 1. genome sequences (in fasta format)
>> 2. Gene prediction (using Fgenesh)
>> 3. creation of GFF3 files
>> 4. uploading in apollo tool
>>
>> what else..
>>
>> looking for you reply.
>>
>> thank you
>> regards
>> Subodh
>>
>>
>>
>> ********************************************************************************
>> Subodh K Srivastava
>> Sr.Lecturer
>> Amity Institute of Biotechnology (AIB)
>> Sce-125, Express way
>> AMITY UNIVERSITY
>> NOIDA-201304
>> UTTAR PRADESH
>> Mob: +91-9911207970
>>
>> --- On Wed, 30/12/09, Dave Clements, GMOD Help Desk <[email protected]> wrote:
>>
>> From: Dave Clements, GMOD Help Desk <[email protected]>
>> Subject: Re: [apollo] Request for annotation protocol using apollo
>> To: "subodh srivastava" <[email protected]>
>> Cc: [email protected]
>> Date: Wednesday, 30 December, 2009, 11:00 PM
>>
>> Hi subodh,
>>
>> Apollo has pretty good documentation that can help you:
>> Apollo Home page:
>>   http://apollo.berkeleybop.org/
>> Apollo User Guide
>>   http://apollo.berkeleybop.org/current/userguide.html
>>   This is a great place to start
>> Step by step tutorial on getting Apollo up and running using a starting 
>> VMware image.
>>   http://gmod.org/wiki/Apollo_Tutorial
>> Links to other tutorials
>>   http://gmod.org/wiki/Apollo
>> You will need to get the Fusarium data into a format that Apollo can read 
>> (see the user guide for supported formats).  GBrowse.org may have some 
>> helpful links:
>>   
>> http://gbrowse.org/wiki/index.php?title=Special%3ASearch&search=fusarium&go=Go
>> Happy new year,
>> Dave C
>> On Tue, Dec 29, 2009 at 9:49 AM, subodh srivastava 
>> <[email protected]> wrote:
>>>
>>> I would like to annotate the Fussarium genome using Apollo. What do I need 
>>> to do?
>>>
>>> may I have the step wise procedure for the annotation and also the file 
>>> format information and place to download.
>>>
>>>
>>>
>>> Thank you
>>>
>>> subodh
>>> ********************************************************************************
>>> Subodh K Srivastava
>>> Sr.Lecturer
>>> Amity Institute of Biotechnology (AIB)
>>> Sce-125, Express way
>>> AMITY UNIVERSITY
>>> NOIDA-201304
>>> UTTAR PRADESH
>>> Mob: +91-9911207970
>>
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>>
>>
>>
>>
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>
>
> --
> ------------------------------------------------------------------------
> Scott Cain, Ph. D.                                   scott at scottcain dot 
> net
> GMOD Coordinator (http://gmod.org/)                     216-392-3087
> Ontario Institute for Cancer Research
>
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>



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