Thanks for the answer, Ed.

An update on that: I tried this on my computer at home running Java 1.5.0 (instead of 1.6.0), an there the coordinates did get filled with the correct values. So I tried the same in the lab, ans again the coordinates were right this time. On the other hand, a Mac Mini here in the lab (running Java 1.5.0) also got the coordinates wrong. So I guess this is a finicky interaction and I'll just have to warn my collaborators to check the coords before submitting any BLAST from Apollo web-start...

Anyway, in all cases (home computer or lab Mac Mini or Linux), the error about the NCBI_BlastOutput.dtd file still occurs after the analysis is (presumably) done.

Thanks
J

Ed Lee wrote:
Hi J,

The NCBI site seems currently down at the moment, so I can't test out
the remote analysis.  But the analysis window from Web Start is
displaying the correct coordinates, so I'm not sure why you're
getting the wrong coordinates...

I'll give more feedback once I can access the qBLAST server.

Cheers,
Ed

On Wed, 6 Jan 2010, J.M.P. Alves wrote:

Hi again,

The problem with the analysis results happens when using the web-start version, but not the standalone local version -- see my email from a few minutes ago for the details on my setup.

When I select one annotation, right click and select "analyze region" on the standalone version, it works perfectly (at least for the NCBI-BLAST analysis, which is all I've tried so far).

When I do the same using the web-start one, some problems occur:

- first, the range is wrong in the genomic region start an end fields, it seems to get only the stop codon coordinates (e.g. 33347 to 33349, instead of the correct 33347 and 36331).

Obnoxious, but not show stopping -- the users can always correct that manually if I warn them beforehand of the problem. But unfortunately it does not stop there.

After correcting the coordinates, I click on run, and Apollo tells me it will take about X seconds to finish.

A while later, it give the following error message:
"Error running analysis: Relative URI "NCBI_BlastOutput.dtd"; can not be resolved without a base URI"

The results do not get added to the analysis panel, I'm assuming they don't even get parsed...

It appears to me that the file in question is sent by NCBI to Apollo, is that right? I have used grep and find on all the Apollo directories I could think of, and found nothing that seemed obvious to me.

Any idea how to solve this? Did I do anything wrong when I created my web-start instance? To compile Apollo for the web-start, all I did was copy my edited chado-adapter.xml, .style, and .tiers files to the conf directory in the source Apollo distribution, and then run "ant jar" from src/java. Did I miss anything?

Thanks
J

--
-------------------------------
João Marcelo Pereira Alves (J)
Post-doctoral fellow
MCV / VCU - Richmond, VA
http://bioinfo.lpb.mic.vcu.edu
f. 1-804-828-3897

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--
-------------------------------
João Marcelo Pereira Alves (J)
Post-doctoral fellow
MCV / VCU - Richmond, VA
http://bioinfo.lpb.mic.vcu.edu
f. 1-804-828-3897

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