Hi Elizabeth, I can't seem to replicate the issue you're having. This is the steps I've followed:
1) Loaded the Genbank file 2) Appended the GFF3 data (with "Embedded FASTA in GFF" checked, since the genomic sequence is already loaded 3) Dragged a transposon from the results track into the annotation track 4) The newly created feature showed both the sequence in "Sequence..." and the data in the "Annotation Info Editor..." I've tried it in both Ubuntu and Mac OSX and they both seem to behave correctly. Genbank files always tend to be a cause of trouble, so if you're successful in loading the GBK file and appending the GFF3 data, I would sugest to then save it to GAME XML format, with both "Save annotations" and "Save evidence (computational results)" checked so that you retain all the data and work with a structured format that Apollo will handle much better. Cheers, Ed On Thu, 20 May 2010, elizabeth henaff wrote:
Hi Ed, I've tried either dragging a result into the annotation panel, and also creating a new annotation, and for both whenever I launch the AIE or the sequence it pulls up a blank window. This is also the case for annotations that are part of the data that I load. I have a gff3 file of results, and a GenBank file of annotations. I've tried various combinations (like, loading the genebank then layering on the gff3, or saving the genbank as chado or game and layering that onto the gff3, or saving both the genbank and the gff3 together as chado or game, and loading them as one) thinking that it might be the data format, but to no avail. To be able to layer on results when I load the genbank data I modified genbank.style and set ShowResults to "true". I've attached the .style and .tiers files and data I'm using, thanks for any help!! cheers, elizabeth On Wed, May 19, 2010 at 10:31 PM, Ed Lee <[email protected]> wrote: Hi Elizabeth, Are you just dragging the features from the results panel into the annotation panel and then trying to launch the AIE? Or are there any steps in between? Perhaps you can provide me with a sample of your data and all the steps you're doing and I'll try to replicate it and help out. Cheers, Ed On Wed, 19 May 2010, elizabeth henaff wrote: Hello! Well, I have successfully managed to load all my computational results into Apollo (thanks Ed) and now I'd like to manually curate these... When I drag a result from the black section to the blue one it makes a new annotation, but when I right-click on this and select Sequence or Annotation Information Editor, a blank window pops up. I'm running version 1.11.12 on Ubuntu. Thanks for any help! Cheers, Elizabeth
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