Hi, if you do:
library("aroma.affymetrix"); setOption(aromaSettings,"memory/ram", 50.0); ram <- getOption(aromaSettings,"memory/ram"); print(ram); do you get NULL or 50.0? My guess is NULL, which would explain it. If so, try to update the aroma framework (minor fixed have been done recently) by: source("http://www.braju.com/R/hbLite.R"); hbInstall("aroma.affymetrix"); BTW, you're running a rather old version of R - I recommend that you update it. Lots of (good) things [http://cran.r-project.org/src/base/NEWS] have changed since. /Henrik On Mon, Aug 29, 2011 at 6:01 AM, feugeas jean-paul <jean-paul.feug...@sls.aphp.fr> wrote: > Hello, > thank you very much for your answer. I have now found a way to increase the > RAM scale factor. > It actually works when I write the RAM scale factor inside the function like > this : > fit(plm,ram=50,verbose=verbose) > But it does not work If I write the RAM scale factor like this : > setOption(aromaSettings,"memory/ram",50.0) > fit(plm,verbose=verbose) > In that case it always takes 1 as RAM scale factor. > Sincerly yours > Jean-Paul > > > For information, here is my sessioninfo() > R version 2.11.0 (2010-04-22) > x86_64-pc-linux-gnu > > locale: > [1] LC_CTYPE=en_US.utf8 LC_NUMERIC=C > [3] LC_TIME=en_US.utf8 LC_COLLATE=en_US.utf8 > [5] LC_MONETARY=en_US.utf8 LC_MESSAGES=en_US.utf8 > [7] LC_PAPER=en_US.utf8 LC_NAME=en_US.utf8 > [9] LC_ADDRESS=en_US.utf8 LC_TELEPHONE=en_US.utf8 > [11] LC_MEASUREMENT=en_US.utf8 LC_IDENTIFICATION=en_US.utf8 > > attached base packages: > [1] stats graphics grDevices utils datasets methods base > > other attached packages: > [1] aroma.affymetrix_2.1.0 aroma.apd_0.1.8 affxparser_1.20.0 > [4] R.huge_0.2.2 aroma.core_2.1.0 aroma.light_1.20.0 > [7] matrixStats_0.2.2 R.rsp_0.5.4 R.cache_0.4.2 > [10] R.filesets_1.0.2 digest_0.5.0 oligo_1.12.2 > [13] oligoClasses_1.10.0 Biobase_2.8.0 R.utils_1.7.5 > [16] R.oo_1.8.0 R.methodsS3_1.2.1 rkward_0.5.3 > > loaded via a namespace (and not attached): > [1] affyio_1.16.0 annotate_1.26.1 AnnotationDbi_1.10.2 > [4] Biostrings_2.16.9 DBI_0.2-5 genefilter_1.30.0 > [7] IRanges_1.6.17 preprocessCore_1.10.0 RSQLite_0.9-4 > [10] splines_2.11.0 survival_2.35-8 tools_2.11.0 > [13] xtable_1.5-6 > >> > > > ----- Message d'origine ----- > De: Henrik Bengtsson <henrik.bengts...@aroma-project.org> > Date: Dimanche, Août 28, 2011 2:02 am > Objet: Re: [aroma.affymetrix] RAM scale factor > À: aroma-affymetrix@googlegroups.com > > >> Hi, >> >> sorry for the delay. This should work, but before diving into serious >> troubleshooting, could you please report your sessionInfo() so we know >> which versions of packages etc you are using? >> >> /Henrik >> >> On Wed, Aug 17, 2011 at 4:38 AM, feugeas jean-paul >> <jean-paul.feug...@sls.aphp.fr> wrote: >> > Hello, >> > I am currently trying to analyze data from 3223 arrays (expression >> arrays, U133 plus 2) with the following code : >> > >> > verbose<-Arguments$getVerbose(-8,timestamp=TRUE) >> > setOption(aromaSettings,"memory/ram",300.0) >> > cdf<-AffymetrixCdfFile$fromFile("/media/lapino/Rabitt/compilation/ThBigCompil/2_arrays/annotationData/chipTypes/HG-U133_Plus_2/HG-U133_Plus_2.cdf") >> > cs <- AffymetrixCelSet$byName("jp", cdf=cdf) >> > bc <- GcRmaBackgroundCorrection(cs,type="affinities"); >> > csB <- process(bc,verbose=verbose); >> > qn <- QuantileNormalization(csB, typesToUpdate="pm"); >> > csN <- process(qn); >> > plm <- RmaPlm(csN, flavor="oligo"); >> > fit(plm,verbose=verbose) >> > >> > at that point, the number of chunks is always 866 as it was with a >> RAM scale factor = 1 although RAM scale factor = 300 as it is >> indicated here : >> > >> > RmaPlm: >> > Data set: jp >> > Chip type: HG-U133_Plus_2 >> > Input tags: GRBC,QN >> > Output tags: GRBC,QN,RMA,oligo >> > Parameters: (probeModel: chr "pm"; shift: num 0; flavor: chr >> "oligo"; treatNAsAs: chr "weights"). >> > Path: plmData/jp,GRBC,QN,RMA,oligo/HG-U133_Plus_2 >> > RAM: 0.01MB >> > 20110817 13:01:43| Started: 20110817 12:17:56 >> > 20110817 13:01:43| Estimated time left: 37862.0min >> > 20110817 13:01:43| ETA: 20110912 20:03:41 >> > 20110817 13:01:43| Fitting chunk #1 of 866...done >> > 20110817 13:01:43| Fitting chunk #2 of 866... >> > 20110817 13:01:43| Units: >> > int [1:62] 1055 1056 1057 1058 1059 1060 1061 1062 1063 1064 ... >> > 20110817 13:01:43| Reading probe intensities from 3223 arrays... >> > 20110817 13:01:43| Identifying CDF cell indices... >> > >> > >> > Is there a mean to reduce the number of chunks ? >> > >> > Thank you in advance for your help >> > >> > Sincerly yours >> > >> > Jean-Paul >> > >> > -- >> > When reporting problems on aroma.affymetrix, make sure 1) to run the >> latest version of the package, 2) to report the output of >> sessionInfo() and traceback(), and 3) to post a complete code example. >> > >> > >> > You received this message because you are subscribed to the Google >> Groups "aroma.affymetrix" group with website http://www.aroma-project.org/. >> > To post to this group, send email to aroma-affymetrix@googlegroups.com >> > To unsubscribe and other options, go to http://www.aroma-project.org/forum/ >> > >> >> -- >> When reporting problems on aroma.affymetrix, make sure 1) to run the >> latest version of the package, 2) to report the output of >> sessionInfo() and traceback(), and 3) to post a complete code example. >> >> >> You received this message because you are subscribed to the Google >> Groups "aroma.affymetrix" group with website http://www.aroma-project.org/. >> To post to this group, send email to aroma-affymetrix@googlegroups.com >> To unsubscribe and other options, go to http://www.aroma-project.org/forum/ > > -- > When reporting problems on aroma.affymetrix, make sure 1) to run the latest > version of the package, 2) to report the output of sessionInfo() and > traceback(), and 3) to post a complete code example. > > > You received this message because you are subscribed to the Google Groups > "aroma.affymetrix" group with website http://www.aroma-project.org/. > To post to this group, send email to aroma-affymetrix@googlegroups.com > To unsubscribe and other options, go to http://www.aroma-project.org/forum/ > -- When reporting problems on aroma.affymetrix, make sure 1) to run the latest version of the package, 2) to report the output of sessionInfo() and traceback(), and 3) to post a complete code example. You received this message because you are subscribed to the Google Groups "aroma.affymetrix" group with website http://www.aroma-project.org/. To post to this group, send email to aroma-affymetrix@googlegroups.com To unsubscribe and other options, go to http://www.aroma-project.org/forum/