hello,have you solved your problem? I met the same error 在 2017年11月14日星期二 UTC+8上午8:29:07,ali jan写道: > > I tried your suggestion but it is still not working: > > > log <- verbose <- Arguments$getVerbose(-8, timestamp=TRUE) > > # Don't display too many decimals. > > options(digits=4) > > > > cdf <- AffymetrixCdfFile$byChipType("CytoScanHD_Array") > > print(cdf) > AffymetrixCdfFile: > Path: annotationData/chipTypes/CytoScanHD_Array > Filename: CytoScanHD_Array.cdf > File size: 612.27 MiB (642007896 bytes) > Chip type: CytoScanHD_Array > File format: v4 (binary; XDA) > Dimension: 2572x2680 > Number of cells: 6892960 > Number of units: 2822125 > Cells per unit: 2.44 > Number of QC units: 4 > > > > gi <- getGenomeInformation(cdf) > > print(gi) > UgpGenomeInformation: > Name: CytoScanHD_Array > Tags: na32,hg19,HB20111108 > Full name: CytoScanHD_Array,na32,hg19,HB20111108 > Pathname: > annotationData/chipTypes/CytoScanHD_Array/CytoScanHD_Array,na32,hg19,HB20111108.ugp > File size: 13.46 MiB (14111425 bytes) > Chip type: CytoScanHD_Array > > > > si <- getSnpInformation(cdf) > > print(si) > UflSnpInformation: > Name: CytoScanHD_Array > Tags: na32,hg19,HB20111108 > Full name: CytoScanHD_Array,na32,hg19,HB20111108 > Pathname: > annotationData/chipTypes/CytoScanHD_Array/CytoScanHD_Array,na32,hg19,HB20111108.ufl > File size: 5.38 MiB (5645047 bytes) > Chip type: CytoScanHD_Array > Number of enzymes: 1 > > > > acs <- AromaCellSequenceFile$byChipType(getChipType(cdf, fullname=FALSE)) > > print(acs) > AromaCellSequenceFile: > Name: CytoScanHD_Array > Tags: HB20111008 > Full name: CytoScanHD_Array,HB20111008 > Pathname: > annotationData/chipTypes/CytoScanHD_Array/CytoScanHD_Array,HB20111008.acs > File size: 170.92 MiB (179217487 bytes) > Number of data rows: 6892960 > File format: v1 > Dimensions: 6892960x26 > Column classes: raw, raw, raw, raw, raw, raw, raw, raw, raw, raw, raw, > raw, raw, raw, raw, raw, raw, raw, raw, raw, raw, raw, raw, raw, raw, raw > Number of bytes per column: 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, > 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1 > Footer: <createdOn>20111108 21:44:22 > PST</createdOn><platform>Affymetrix</platform><chipType>CytoScanHD_Array</chipType><srcFile><filename>CytoScanHD_Array.probe_tab</filename><filesize>409176930</filesize><checksum>beaaa56413608b3f9d9d464a07cddcae</checksum></srcFile><createdBy><fullname>Henrik > > Bengtsson</fullname><email>henrik.bengts...@aroma-project.org > <javascript:></email></createdBy> > Chip type: CytoScanHD_Array > Platform: Affymetrix > > > > csR <- AffymetrixCelSet$byName("CNV", cdf=cdf) > > print(csR) > AffymetrixCelSet: > Name: CNV > Tags: > Path: rawData/CNV/CytoScanHD_Array > Platform: Affymetrix > Chip type: CytoScanHD_Array > Number of arrays: 460 > Names: GSM1317174_H050150N-CytoScanHD_515-H050150N, > GSM1317175_H050150T-CytoScanHD_516-H050150T, > GSM1317176_H050154N-CytoScanHD_521-H050154N, ..., > GSM1317635_H112971T-CytoScanHD_831-H112971T [460] > Time period: 2012-03-12 16:49:41 -- 2012-04-05 08:47:43 > Total file size: 29.55 GiB > > > > cs <- csR > > par(mar=c(4,4,1,1)+0.1) > > plotDensity(cs, lwd=2, ylim=c(0,0.40)) > > > acc <- AllelicCrosstalkCalibration(csR, model="CRMAv2") > > print(acc) > AllelicCrosstalkCalibration: > Data set: CNV > Input tags: > User tags: * > Asterisk ('*') tags: ACC,ra,-XY > Output tags: ACC,ra,-XY > Number of files: 460 (29.55 GiB) > Platform: Affymetrix > Chip type: CytoScanHD_Array > Algorithm parameters: {rescaleBy: chr "all", targetAvg: num 2200, > subsetToAvg: int [1:6538333] 1 2 3 4 5 6 7 8 11 12 ..., mergeShifts: logi > TRUE, B: int 1, flavor: chr "sfit", algorithmParameters:List of 3, ..$ > alpha: num [1:8] 0.1 0.075 0.05 0.03 0.01 0.0025 0.001 0.0001, ..$ q: num > 2, ..$ Q: num 98} > Output path: probeData/CNV,ACC,ra,-XY/CytoScanHD_Array > Is done: FALSE > > print(csC) > Error in print(csC) : object 'csC' not found > > sessionInfo() > R version 3.4.2 (2017-09-28) > Platform: x86_64-w64-mingw32/x64 (64-bit) > Running under: Windows >= 8 x64 (build 9200) > > Matrix products: default > > locale: > [1] LC_COLLATE=English_United States.1252 LC_CTYPE=English_United > States.1252 LC_MONETARY=English_United States.1252 > [4] LC_NUMERIC=C LC_TIME=English_United > States.1252 > > attached base packages: > [1] stats graphics grDevices utils datasets methods base > > other attached packages: > [1] aroma.light_3.8.0 aroma.affymetrix_3.1.0 aroma.core_3.1.1 > R.devices_2.15.1 R.filesets_2.11.0 R.utils_2.5.0 > [7] R.oo_1.21.0 affxparser_1.50.0 R.methodsS3_1.7.1 > > loaded via a namespace (and not attached): > [1] matrixStats_0.52.2 codetools_0.2-15 listenv_0.6.0 future_1.6.2 > digest_0.6.12 R.huge_0.9.0 PSCBS_0.63.0 > [8] tools_3.4.2 R.cache_0.12.0 parallel_3.4.2 > compiler_3.4.2 base64enc_0.1-3 aroma.apd_0.6.0 R.rsp_0.41.0 > [15] globals_0.10.3 DNAcopy_1.52.0 > > traceback() > No traceback available > > On Wednesday, January 13, 2016 at 11:05:11 PM UTC+5, Henrik Bengtsson > wrote: >> >> On Sun, Jan 10, 2016 at 11:36 PM, <wxp...@gmail.com> wrote: >> > >> > Hi dear professor Henrik Bengtsson, >> > >> > When I was declaring the raw data set of CytoScan HD Array with >> aroma.affymetrix, I'm encountering the following errors: >> > >> > "Error: object 'csC' not found." >> >> It seems like you didn't run the command before. Make sure each >> object is actually created in every step by printing it, e.g. >> print(cdf), etc. That should help you narrow down where your problem >> is. >> >> /Henrik >> >> > >> > I have searched the forum, unfortunately, I can't solve this problem. >> > >> > Could you give me some suggestions and help me solve it ? >> > >> > The following are the R code of mine: >> > >> > > setwd("G:/") >> > >> > >library("aroma.affymetrix") >> > >> > >log <- verbose <- Arguments$getVerbose(-8, timestamp=TRUE) >> > >> > >options(digits=4) >> > >> > >cdf <- AffymetrixCdfFile$byChipType("CytoScanHD_Array") >> > >> > >gi <- getGenomeInformation(cdf) >> > >> > >si <- getSnpInformation(cdf) >> > >> > >acs <- AromaCellSequenceFile$byChipType(getChipType(cdf)) >> > >> > >csR <- AffymetrixCelSet$byName("CytoScanHD_Array", cdf=cdf) >> > >> > >acc <- AllelicCrosstalkCalibration(csR, model="CRMAv2") >> > >> > >csC <- process(acc, verbose=verbose) >> > >> > > print(csC) >> > >> > > sessionInfo() >> > R version 3.2.2 (2015-08-14) >> > Platform: x86_64-w64-mingw32/x64 (64-bit) >> > Running under: Windows 7 x64 (build 7601) Service Pack 1 >> > >> > locale: >> > [1] LC_COLLATE=English_United States.1252 >> > [2] LC_CTYPE=English_United States.1252 >> > [3] LC_MONETARY=English_United States.1252 >> > [4] LC_NUMERIC=C >> > [5] LC_TIME=English_United States.1252 >> > >> > attached base packages: >> > [1] stats graphics grDevices utils datasets methods base >> > >> > other attached packages: >> > [1] aroma.light_2.4.0 aroma.affymetrix_2.14.0 affxparser_1.42.0 >> > [4] aroma.core_3.0.0 R.devices_2.13.2 R.filesets_2.10.0 >> > [7] R.utils_2.2.0 R.oo_1.19.0 R.methodsS3_1.7.0 >> > >> > loaded via a namespace (and not attached): >> > >> > [1] matrixStats_0.50.1 codetools_0.2-14 listenv_0.6.0 >> future_0.10.0 >> > [5] digest_0.6.8 R.huge_0.9.0 PSCBS_0.60.0 >> tools_3.2.2 >> > [9] R.cache_0.12.0 parallel_3.2.2 base64enc_0.1-3 >> aroma.apd_0.6.0 >> > [13] R.rsp_0.21.0 globals_0.6.0 DNAcopy_1.42.0 >> > >> > CEL format raw data are locate in >> "G:/rawData/CytoScanHD_Array/CytoScanHD_Array" >> > >> > Looking forward your replay. Thank you very much. >> > >> > >> > >> > >> > >> > >> > >> > >> > -- >> > -- >> > When reporting problems on aroma.affymetrix, make sure 1) to run the >> latest version of the package, 2) to report the output of sessionInfo() and >> traceback(), and 3) to post a complete code example. >> > >> > >> > You received this message because you are subscribed to the Google >> Groups "aroma.affymetrix" group with website >> http://www.aroma-project.org/. >> > To post to this group, send email to aroma-af...@googlegroups.com >> > To unsubscribe and other options, go to >> http://www.aroma-project.org/forum/ >> > >> > --- >> > You received this message because you are subscribed to the Google >> Groups "aroma.affymetrix" group. >> > To unsubscribe from this group and stop receiving emails from it, send >> an email to aroma-affymetr...@googlegroups.com. >> > For more options, visit https://groups.google.com/d/optout. >> >
-- -- When reporting problems on aroma.affymetrix, make sure 1) to run the latest version of the package, 2) to report the output of sessionInfo() and traceback(), and 3) to post a complete code example. You received this message because you are subscribed to the Google Groups "aroma.affymetrix" group with website http://www.aroma-project.org/. To post to this group, send email to aroma-affymetrix@googlegroups.com To unsubscribe and other options, go to http://www.aroma-project.org/forum/ --- You received this message because you are subscribed to the Google Groups "aroma.affymetrix" group. To unsubscribe from this group and stop receiving emails from it, send an email to aroma-affymetrix+unsubscr...@googlegroups.com. For more options, visit https://groups.google.com/d/optout.