Hello --
Thank you for your tip.
However, I can't postpone my work. I need an answer now.
Quite a bit needs to be done in terms of documentation.
Best regards,
Henry Lenzi
On Wed, 2002-08-14 at 05:45, Murphy, Terry wrote:
> Henry
>
> You might be interested to know that the SGM and Sanger Centre are running
> four one day workshops between now and the end of the year, with emphasis
> on Artemis and ACT. The latter may be of interest, as it might serve to
> address your questions. The web page is
> www.socgenmicrobiol.org.uk/MTGPAGES/bioinformatics.htm
> although you need to be an SGM member to participate.
>
> Kind regards
>
> Dr Terry Murphy
> Brucella Research
> Department of Bacterial Diseases
> Veterinary Laboratories Agency
> Woodham Lane
> New Haw
> ADDLESTONE
> Surrey
> KT15 3NB
> Tel: +44 (0)1932 357891
> Fax: +44 (0)1932 357423
> E-mail: [EMAIL PROTECTED]
>
>
> -----Original Message-----
> From: Henry Lenzi [mailto:[EMAIL PROTECTED]]
> Sent: 13 August 2002 19:45
> To: artemis-users
> Subject: [artemis-users] Questions regarding ACT - what do you do after
> you MSPcrunch the data?
>
>
>
> Hello --
>
> I've read a tutorial on ACT that says you need to have, e.g.,
>
> tbrucei.fasta
> tcruzi.fasta
> tbrucei.embl
> tcruzi.embl
>
> Then it says you have to process the fasta files by formating the
> files
> with
>
> $formatdb -i t.cruzii -p F
>
> then blastall
>
> $blastall -t brucei.fasta -d tcruzi.fasta -p tblastx -o myblast1.out
>
> And finally, MSPcrunch them:
>
> $MSPcrunch -d -I50 myblast.out > myblast.crunch
>
>
> What are the annotated (embl) files for?
>
> I feel that the chapter on the tutorial explaining how to make a
> comparison file is way too short. This should be very thoroughly
> explained since it a crucial step...
>
> Thank you,
>
> Henry
>
>
