Many thanks to all who replied to my posting. The problem was solved, following advice of Tim Carver and David Bauer, by deleting features at the ends of the sequence which referenced other sequences e.g.

FT   gene            complement(AC012146.25.1.171309:6053..52695)

Regards,

Lesley Nicolson

Glasgow uni

 


From: Lesley Nicolson [mailto:[EMAIL PROTECTED]
Sent: 22 June 2006 17:21
To: '[email protected]'
Subject: problem importing ensembl export view flat files into Artemis

 

Hi – I would really appreciate help with a problem importing files from ensembl export view (http://www.ensembl.org/Homo_sapiens/exportview).

 

I’ve exported human chromosomal sequence (500Mb) from ensembl export view, with gene, vega and genscan features, as a flat file (saved as text file). In notepad this looks comparable to files that I can open in artemis (apart from the XX blank lines – but it makes no difference removing these):  example:

ID   17    standard; DNA; HTG; 500000 BP.

XX

AC   chromosome:NCBI36:17:10050467:10550466:1

XX

SV   chromosome:NCBI36:17:10050467:10550466:1

XX

DT   21-JUN-2006

XX

DE   Homo sapiens chromosome 17 NCBI36 partial sequence 10050467..10550466

DE   annotated by Ensembl

XX

KW   .

XX

OS   Homo sapiens (human)

OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;

 

Regards,

 

Lesley Nicolson

Glasgow Veterinary School

Div I&I, Level 4 Sir Henry Wellcome Building

 

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