> > > I want to use artemis to show the transcription start sites in a > bacterial genome. Is there anyone who can tell me how to do that? > > Are the TSSs annotated in your genome? Are even the genes/CDS annotated in your genome? Or are you expecting Artemis to magically find them for you?
Although putative CDS are "easy" to find in bacteria, the correct start codon is not always obvious (especially with atypical RBS). The TSS is even more difficult to determine. -- --Dr Torsten Seemann --Victorian Bioinformatics Consortium, Monash University, AUSTRALIA --http://www.bioinformatics.net.au/
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