It seems the big_blast.pl script cannot find the blastn executable. Do you have blastn somewhere? If not, download the old blast-package (not blast+) from NCBI, unpack it, and place the blastn executable in a folder that is in your path, i.e., a place where scripts or programs can find it. You probably have a folder called /usr/bin/, try placing it there. Type env (using a terminal window) to see your path (and a lot of other information).
Cheers, Henrik ________________________________________ Från: artemis-users-boun...@sanger.ac.uk [artemis-users-boun...@sanger.ac.uk] för Tony Tang [ttony...@gmail.com] Skickat: den 28 april 2011 22:56 Till: email@example.com Ämne: [Artemis-users] big_blast Hi All, Does any body know how to set up environment for running big_blast.pl<http://big_blast.pl>? I have installed "Pathogen Sequencing Unit internal perl modules" and copied big_blast.pl<http://big_blast.pl> into folder modules. There is an error when I was trying to run the big_blast.pl<http://big_blast.pl>. "Can't exec "blastn": No such file or directory at ./big_blast.pl<http://big_blast.pl> line 489, <IN> line 2." Any help will be appreciated. Thanks, Tony _______________________________________________ Artemis-users mailing list Artemisfirstname.lastname@example.org http://lists.sanger.ac.uk/mailman/listinfo/artemis-users