Hi Bob
Thanks for your explanations.
It's seem the thing to do is scape this column or maybe make a script to 
remove this Error string from my .gpr's...
In any case, the problem with this M colum is also not a big one. I do 
normally my data statistical analysis in R. To have my data processing from 
BASE is an interesting possibility and this is something for sure I want to 
explore in the future but I haven't reached this stage yet. My believe was 
that this is possible -at least I did some trials with BASE1 some time ago- 
and also the connection between R and BASE. By now, I am very happy to have 
a centralized repository for the microarray data in my insitution and I 
really have to thank the BASE development team for this great contribution.

As far as I could see, you have separate pluings for Cy3/Cy5 and Cy5/Cy3 
data import...

Cheers
Ana


On Fri, 2 Mar 2007 17:51:20 +0000, Bob MacCallum wrote
> Ana Conesa writes:
>  > <html>
>  > <body>
>  > Hi David,<br><br>
>  > Yes I thought of this option but I am not sure I want this. (I 
> mean, I am > sure I don't). Other option is to skip this column from 
> the importer (a > pity for the M value) and compute this in a later 
> step. I am not yet that > far in BASE, so I do not know at the 
> moment how you do this, but I expect > this will be possible. <br><br>
> 
> Hi Ana,
> 
> This may be of some help to you:
> 
> Hypothetically, if this was happening with our BASE1 instance we 
> would safely be able to ignore the M value column completely.  Any 
> dual channel calculations (e.g. ch1/ch2 ratios etc) in the GenePix 
> files tend to be redundant in BASE because
> 
> a) BASE (1) imports dye swap experiments with different importers so 
> that the experimental/control data always ends up in channel 
> 1/channel 2.
> 
> b) once you've normalised the data in BASE, the original genepix ratios
> are no longer valid.
> 
> I haven't loaded any data in our BASE2 test server yet (data 
> migrated from BASE1 only) - so I have a general question: what is 
> the strategy for loading dye swaps in BASE2, is it the same as BASE1?
> 
> cheers,
> Bob.
> 
> -- 
> Bob MacCallum | VectorBase Developer | Kafatos/Christophides Groups |
> Division of Cell and Molecular Biology | Imperial College London |
> Phone +442075941945 | Email [EMAIL PROTECTED]
> 
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