See my previous reply to this error.

http://sourceforge.net/mailarchive/message.php?msg_name=462CAC84.7020801%40thep.lu.se

/Nicklas

Jean-Pierre Gauthier wrote:
> Hi BASEr's
> 
> I have some trouble to perform my analysis thus I send you
> what I have done.
> Could somebody say me what is wrong?
> We are using BASE 2.2.1
> and Mysql Ver 14.7 distrib 4.1.12
> thanks
> 
> Following the Getting started User documentation:
> Create reporters
>  >Raw data importer: from genepix .gpr files like this
> 
> Block   Column  Row     Name    ID      X       Y       Dia.    F635 
> Median     F635 Mean       F635 SD F635 CV B635    B635 Median     B635 
> Mean       B635 SD B635 CV % > B635+1SD    % > B635+2SD    F635 % 
> Sat.     F532 Median     F532 Mean       F532 SD F532 CV B532    B532 
> Median     B532 Mean       B532 SD B532 CV % > B532+1SD    % > 
> B532+2SD    F532 % Sat.     Ratio of Medians (635/532)      Ratio of Means 
> (635/532)        Median of Ratios (635/532)      Mean of Ratios 
> (635/532)        Ratios SD (635/532)     Rgn Ratio (635/532)     Rgn R2 
> (635/532)        F Pixels        B Pixels        Circularity     Sum of 
> Medians (635/532)        Sum of Means (635/532)  F635 Median - 
> B635      F532 Median - B532      F635 Mean - B635        F532 Mean - 
> B532        F635 Total Intensity    F532 Total Intensity    SNR 635 SNR 532 
> Flags   Normalize       Autoflag
> 1       1       1       aphl3sd-xxxi-h6 
> 1a      2080    2590    110     3077    3005    713     23      61      61 
>      76      88      115     100     100     0       6165    5868    1449 
>   24      171     171     177     55      31      100     100     0 
> 0.503   0.517   0.509   0.514   1.42    0.496   0.833   80      656     100 
>      9010    8641    3016    5994    2944    5697    240388  469469  33.284 
>   103.473 0       0       0
> 1       2       1       Ap_SNI1_1_A04_T7        15a     2380    2610    100 
>      2220    2216    364     16      61      61      68      34      50 
>   100     100     0       2271    2235    334     14      168     168 
> 171     47      27      100     100     0       1.027   1.043   1.026 
> 1.042   1.291   1.055   0.838   80      560     100     4262    4222 
> 2159    2103    2155    2067    177291  178813  63.176  43.915  0       0 
>      0
> ....
> 10080 lines
> 
>  >create ok
> 
>  >reporter importer
> File
> Block   Column  Row     ID      Name    Description
> 1       1       1       1a      aphl3sd-xxxi-h6 (Q76LV1) 90-kDa heat shock 
> protein beta
> 1       2       1       15a     Ap_SNI1_1_A04_T7        NONE
> ....
> 10080 lines
>  >ok
> 
>  >reporter map importer
> Same file than before
>  >ok
> 
> Uploading files ok
> reporter import ok
> reporter map import ok
> project ok
> raw bioassays ok
> experiment + raw bioassays ok
> 
> the error comes when I try to create a root bioassay set
>   all seems to work well (Base say calculating spot intensities 10 of 12 
> raw bioassay(s) done).
> same pb with 1 or 2... raw bioassay.
> And then error Invalid use of GROUP clause .. (in french).
> For my last retry I suppress the  Log Ratio (635/532) column to avoid 
> *Error* an to have
> the same lines number in all files...
> 
> Here is the log.
> net.sf.basedb.core.BaseException Utilisation invalide de la clause GROUP
> at net.sf.basedb.core.AbstractSqlQuery.iterate(AbstractSqlQuery.java193)
> at net.sf.basedb.core.DataCube.countSpotMappings(DataCube.java682)
> at net.sf.basedb.core.DataCube.onBeforeCommit(DataCube.java171)
> at net.sf.basedb.core.DbControl.commit(DbControl.java365)
> at 
> net.sf.basedb.plugins.IntensityCalculatorPlugin.run(IntensityCalculatorPlugin.java261)
> at 
> net.sf.basedb.core.PluginExecutionRequest.invoke(PluginExecutionRequest.java88)
> at net.sf.basedb.core.InternalJobQueue$JobRunner.run(InternalJobQueue.java420)
> at java.lang.Thread.run(Thread.java595)
> Caused by java.sql.SQLException Utilisation invalide de la clause GROUP
> at com.mysql.jdbc.SQLError.createSQLException(SQLError.java946)
> at com.mysql.jdbc.MysqlIO.checkErrorPacket(MysqlIO.java2870)
> at com.mysql.jdbc.MysqlIO.sendCommand(MysqlIO.java1573)
> at com.mysql.jdbc.MysqlIO.sqlQueryDirect(MysqlIO.java1665)
> at com.mysql.jdbc.Connection.execSQL(Connection.java3176)
> at 
> com.mysql.jdbc.PreparedStatement.executeInternal(PreparedStatement.java1153)
> at com.mysql.jdbc.PreparedStatement.executeQuery(PreparedStatement.java1266)
> at 
> com.mchange.v2.c3p0.impl.NewProxyPreparedStatement.executeQuery(NewProxyPreparedStatement.java76)
> at net.sf.basedb.core.AbstractSqlQuery.iterate(AbstractSqlQuery.java184)
> ... 7 more
> Job parameters
> Experimentlsd_experiment
> FormulaMean FG
> Bioassay set nameNew bioassayset
> Raw bioassaysh1ld_cy3, h1ld_cy5, h1sd_cy3, h1sd_cy5, h2ld_cy3, h2ld_cy5, 
> h2sd_cy3, h2sd_cy5, h3ld_cy3, h3ld_cy5, h3sd_cy3, h3sd_cy5
> 
> 
> 
> Gauthier Jean-Pierre.
> Bio-informatique.
> INRA. UMR BIO3P.
> Domaine de la Motte. BP. 35327.
> 35653 LE RHEU CEDEX.
> tel  : 33(0)2.23.48.51.68
> fax  : 33(0)2.23.48.51.50
> mail : [EMAIL PROTECTED]
> 
> 
> 
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