Allan Balliett wrote: Boy, Elaine - This is a good one!!!

>  At 8:32 AM -0500 1/9/02, ron poitras wrote:
>  >For those of us still dowsing impaired, lab tests are important in assessing
>  >results. The cost of testing compost tea to determine the diversity of
>  >microbial life and the effectiveness of various additions to the brew can be
>  >a barrier to perfecting a compost tea product. You can't always wait until
>  >your plants are giving you signals. Does Dr Ingram have any insights on
>  >simple tests that could easily be performed at the farm for not much cost to
>  >determine quality of a batch of compost tea?

Yes, this is a good one.  I'm very involved in this exact question. 
In fact, one
tea maker has stopped talking to me because I keep insisting that he 
have data on
whether his tea maker actually makes tea.  If the brown water his machine makes
doesn't have the organisms in it, then I will contest that it isn't tea.

So, when you buy a machine, you need to have the tea maker show you 
that they can
document that the machine is at least CAPABLE of making a tea with high numbers
in it.  They should show you a count of bacterial biomass, fungal 
biomass at the
very least.  ACTIVE bacteria and fungi would be even better becasue 
then you know
the organisms will start helping your plants right away.  Plate counts don't
really help here becasue dormant, sleeping organisms that may or may 
not ever do
anything in soil get included in the count, but most of the organisms that are
doing things in soil or on leaf surfaces can't even begin to grow in the plate
medium.  Plate count are useful for other things, however, so don't 
get me wrong
that you should never consider them.  They just aren't useful in answering this
question.

So, get the data from the machine maker.  Then, you want to think of one of the
following ways to assess your tea:

1.  Total and active bacteria and fungi:  This test takes about 20 
minutes in the
lab.  So, about 2 hours after the sample arrives at SFI, you are going to get
your results back.  The test is $80, minus a discount if you send the 
submission
form and payment in with the sample, if I remember correctly.  You know if the
tea has the number of organisms needed to result in disease suppression.

2.  Oxygen concentration;  As aerobic organisms grow, they use 
oxygen.  Thus, if
you measure oxygen concentration in the tea, you can follow the growth of the
bacteria and fungi.  This is really useful, right now data.  Oxygen probes are
spendy though.  From $500 to $2000 dollars.  Look at the Woods End 
Lab web site,
or the YSI website.

3.  Temperature.  Increase in temperature means the bactea and fungi 
are growing!

4.  Pathogen inhibition:  This takes several weeks to get the information back,
but does give you an idea if you are getting the disease suppressive organisms
into the tea and having them work in lab conditions.  There's always a problem
knowing if the same thing will happen in field conditions, so it is only an
indicator.  The test is spendy depending on which pathogens you test, 
contact BBC
Labs.

5.  Diversity index:  Six different plate media are used, and it relates to how
diverse the bacteria are, although again, a small percentage of the species are
actually assessed.  Consider that soil should have, oh, 25,000 species of
bacteria PER TEASPOON.  On these plates, perhaps a maximum number of 
250 species
can grow.  So, not a huge percentage of the possible species come up. 
Again, in
the lab, so what is the relation between what grows on the plates and will do
it's thing in soil?  Don't know.  But, maybe an indicator.  The work that BBC
Labs needs to do is show what it means if the index has a value of 3, or 13, or
33.  You have more species if the index is 33, but does that mean you have
disease suppression, or will improve soil structure and cut water loss?  Don't
know that.
But could be interesting.  This test is $90.

Also measure foam, color, smell.  I think if you record temperature, 
color of the
tea, smell, and foam, and learn what the tea normally looks and 
smells like, you
can use these to know if the tea is a good one.  I like to measure total and
active bacteria and total and active fungi on the first three teas I make each
spring, and get the smell and color down again.  As long as the smell, color,
foam, etc are the same with each run, then I know my organism numbers.  If the
tea is different, then I test it to make sure it is ok.

Bio-film formation in machines is a big problem.  Anaerobic anything 
is a serious
problem - you will have pathogens sooner or later if you allow anaeorbic
conditions.

Hope this helps!

Elaine Ingham

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