Allan Balliett wrote:

>  Elaine, you asked the poster to make their questions about OSU more focused:
>
>  >What I would like to know is:
>  >
>  >1) What bacterium is applied to the soil to breakup the hardpan ?
>  >
>  >2) What medium is the carrier for the bacteria ? (water, sawdust, granules)
>  >
>  >3) What are the equipment requirements to apply the bacteruim to the soil ?
>  >     (sprayer, spreader, field broadcaster or a cannon)
>  >
>  >4) Which department at "THE" Ohio State University was the sponsor of the
>  >study.  (microbiology, plant and soil chemistry, the football team)
>  >
>  >Thank You in advance for your kind indulgence.

Thank you for being more specific.  These are more answerable questions!

There are several single species of bacteria that can be used as inocula to do
any specific job.  But go back to first principles.  Each bacteiral species has
been selected to function best in a specific set of conditions.  There are
almost no "universal" bacteria that function at all temperatures, all 
moistures,
etc.  Soil always has some set of bacterial species functioning, so people may
miss-conclude that there are just a few species functioning all the time, but
this is not the case.   Ask any microbiologist. As you go through the course of
a day, much less a week, much less a month, certain species of 
bacteria (applies
to fungi, and protozoa as well, maybe daily for nematodes, but certainly weekly
for nematodes and microarthropods) wake up, do their thing by being 
most able to
out-compete everything else, and then go back to sleep because conditions are
not right for it to compete.

So, there is NO SINGLE SPECIES of bacteria that you can apply that will be
successful at performing any function through the whole summer.  A single
species inoculum might be beneficial for a day, or a week, or a month, until
other bacteria arrive and start competing with it.  Therefore, what we need to
do is apply the FULL spectrum of species of bacteria, fungi, protozoa and
nematodes that the soil needs to break up hardpan.

What is it that supplies this full spectrum?

Well, back up a second, how many species in the full spectrum?  Work by Jim
Tiedje, Michael Klug, their graduate students (Michgan State, Center for
Microbial Ecology), work by Janice Thies (Cornell), and others show that, using
DNA analysis, there are probably over 25,000 species of bacteria in a teaspoon
of soil - ah, healthy soil.  Important distinction there.

If you've hammered your soil with toxic chemicals (i.e., pesticides), salts
(otherwise known as inorganic fertilizers), and constant plowing, you've
destroyed the health of your soil.  How do you know?  Because the species of
bacteria, fungi, protozoa and nematodes have been severely reduced, and the
species that are left are the pests and pathogens.  Why?  Becasue pests and
pathogens have been selected for the boom and bust life style common to the
majority of pathogens and pests.  Get the normal organisms back in 
the soil that
compete with the pathogens and pests, and disease is reduced.

How many species are required?  Thousands.  Probably more than 25,000 
species of
bactera, but it's a good place to start.  Then you probably have MOST of the
species needed.  But do you have ALL the species needed?  That's where
isolations become important.  Send a soil, or compost, or compsot tea into BBC
Labs to do the pathogen inhibition test.  If for some reason you don't have the
species needed to take out the pathogen in a lab incubation, it is likely (but
not guaranteed) that you lack the important species in the field.  Good
indication that you need to add more compost, or tea, or even buy the 
critter in
an inoculum off the shelf.

The same logic applies to breaking up hardpan.  Do you have the bacterial
species that make the glues to bind the clays, sand, silt, OM, roots, etc?  The
fungi that make the foundation walls for soil structure, commonly called
macroaggregates.  Do you have the engineers in your soil that make pores?  Hold
water?  Allow oxygen to diffuse into the soil?  You may need to add some of
these organisms.

How do you add this huge diversity required?  Are you going to buy single
species inocula in order to do this?  You don't have enough money to buy 25,000
single species, much less we don't have that many of the species isolated and
grown in lab media to be able to make them to sell to you!

So how do you get that diversity?  It's called compost.  Locally made, with the
species of bacteria, fungi, protozoa and nematodes already adapted to your
soil.  If you buy bacteria from Japan, guess what?  They are not  going to
survive.  What I like to say is, Japanese bacteria require sushi, and there is
no sushi in American soils.  So, you want the same functional groups, but you
want those species adapted to your soils, your climate, your plant cultivars.

Now, what about the case where most of the "good guys" have been killed in your
soil?  If you add "foreign" species, can you see a good response?  Of course.
There are no better-adapted species already present.  So, in really poor soil,
addition of any organism is going to help.  But, they won't survive.  Why not
add, from the very beginning, the whole diversity of good guys that 
will survive
and grow?  You may need to come back and add more compost from time-to-time to
maintain food resources for that diversity, to add in species that didn't
survive the first time around that the compost was applied.

If the compost available is too far away, or too expensive, and making your own
compost is too much trouble, then making compost tea becomes very reasonable.
It will have the same species as in your compost, only much more transportable.
You only need 15 pounds of compost to make 500 gallons of great tea 
(ok, given a
good tea making machine as well - see the Compost Tea Brewing Manual 
for all the
details).  Apply at 5 gallons to the acre.  Easy.

But, you don't get the long term food resources with a composttea that you get
in solid compost.  The way I think of it is that compost tea supplies the
organisms and the food to maintain those organisms for 3 to 4 months.  Compost
supplies the organisms and the food to maintain those organisms for 4 to 5
years.

So, what bacterium is supplied to break up hardpan?  There are about 8,000
species of bacteria that you need.  What are their names?  They don't have
names, just ID numbers, for the most part.  I don't think most people 
would find
me the DNA sequences for those bacteria very useful, and I'm not 
absolutely sure
that those bacteria are the right ones for your soil, climate, plants species,
etc.  So, I'll pass on giving you that info, ok?  Maybe in 20 years we'll have
that data.  And realize, it's not just me doing that work.  The folks at Duke
University are probably actually the ones in the forefront on that right now.
And Cornell.  And maybe UC Berkeley.

Medium to carry the inoculum?  Compost, or compost tea.  Mix the compost about
30% with sand (70%) and as you deep rip (to get to 4 feet for example), add the
mix into the crack.  Over apply compost tea in a drip line behind the blades.
If you can't rip, then do cores as deep as you can go and re-fill with the mix
of compost and sand, or inject the tea into the soil at teh right depth.  The
trick there is to make sure there's good aeration that deep.  Leave an "air
line" down into the soil.

Oh-h-h-h, getting nasty, "THE" Ohio State University.  You mean "the OTHER
OSU"?  Please realize you are speaking to someone from Oregon State University.
The other guys are doing a good job, but just can't really compete with us!

OK, enough ribbing.  If you go to the Ohio State University's website, you
should be able to locate Harry Hoitink's department.  I don't have that
information memorized, sorry, it just isn't as important to me as remembering
the work that he's contributed over the years.  His department is probably
something like Plant Pathology, or Horticulture.  If you go to the ATTRA
website, and look up their page on COMPOST, Harry's name comes up all over the
place.  From those references you should be able to find his department at the
other OSU.  So, go have fun.

Hope this answers your questions!

Elaine Ingham

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