I've been reading with great interest since Bioc-sig-seq started, we finally
have a Solexa GA2... but still in the box so I have no hands on experience
yet...
Must admit I've not sorted out in my head what parts of the analysis are most
suited R (my preference is as much as possible by but I understand some of the
limitations...)
Anyway the new hardware comes with a cluster of PCs (IPAR) that does the image
analysis and they promise will do the base calling soon (not alignment?)
Given this, and that we like using R when possible, and that we have some
modest resources, can someone comment between the suggested server for the GA2
system: quad Xeon 7000 series with 32GB Ram verses a quad Xeon box with the
5000 series processor (3 GHz). We have some experience setting up the 5000
series box which is about ½ the cost of the 7000 series. We do have High
Performance computing at an off-site location but transferring LARGE files to
that location may be an issue...so would like to do as much in-house as
possible.
Do you find that the analysis you have done benefits greatly from the faster
processor or would 2x 5000 boxes by better option in your opinion? Any comments
on the amount of memory per processor, I was thinking that perhaps this should
be higher than recommended if we like to use R? These things I need to resolve
before I use the sequencer unfortunately. I see in some posts that people are
using machines with 64GB of memory, is that typical?
Also if anyone with real word experience can comment on the typical size of the
alignment file (for paired end reads on a good day), that is the
s_N_export.txt file generated by ELAND and the s*_sequences.txt file generated
by GERALD that would be helpful too (I have the standard product info). Are
there other files generated by the pipeline that you have found particularly
useful in downstream analysis or that are useful in other 3rd party
applications that you have tried?
Thanks in advance
Paul
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