Hi everyone,
Although this question is more related to ChIP and ChIP-seq, it could be
probably anchored in a more general statistical context.
The question is : what method is better to assess the significance of the
change in a signal (the signal can be DNA binding, for instance) given the
background and 2 conditions.
<. condition1 (eg no treatment) : background = 1;
signal = 5;
<. condition2 (eg hormonal treatment) : background = 3;
signal = 6.
The methods can be :
a. substract the background : i.e. (signal_treatment - background_treatment)
/ (signal_no_treatment - background_no_treatment)
b. calculate the fold change: i.e. (signal_treatment / background_treatment)
/ (signal_no_treatment / background_no_treatment)
c. any other method ? i.e. (signal_treatment - signal_no_treatment) / (
background_treatment - background_no_treatment)
Thank you very much.
Bogdan
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