On Mon, Oct 12, 2009 at 1:45 PM, joseph <[email protected]> wrote:

> Hello
> What do I need to do to SimpleRleList and Rle before I use them to make a
> track with export().
> I tried to find help in an earlier thread (WIG from coverage), but I am
> still not able to solve the problem.
>
> > cvge_s_1
> SimpleRleList: 25 elements
> names(25): chr1 chr10 chr11 chr12 chr13 chr14 ... chr7 chr8 chr9 chrM chrX
> chrY
> > cvge_s_1$chr5
>  'integer' Rle instance of length 180857866 with 15165 runs
>  Lengths:  181838 20 27419 21 25146 21 18711 19 18247 13 ...
>  Values :  0 1 0 1 0 1 0 1 0 1 ...
>
>
> export(cvge_s_1$chr1, "cvge_s_1_chr1.wig")
> #This is what I get for chr1:
> track name="R Track" type=wiggle_0
> variableStep chrom=chr1 span=1
> 558223    1
> 558249    1
> 558777    1
> 559783    2
> 559784    3
> ........
>
> export(cvge_s_1$chr5, "cvge_s_1_chr5.wig")
> #This is what I get for all the other chromosomes; it is always chr1:
>
> track name="R Track" type=wiggle_0
> chr1    0    4948    0
> chr1    4948    4949    2
> chr1    4949    4950    3
> chr1    4950    4970    4
> chr1    4970    4971    3
> chr1    4971    4972    2
>
>
Yes, it will always be chromosome 1, because that is the default when
creating a RangedData from an Rle. When extracting "chr5" element from the
RleList, you're losing the chromosome information. Instead, just subset,
e.g. cvge_s_1["chr5"].


> > sessionInfo()
> R version 2.10.0 alpha (2009-10-01 r49909)
> x86_64-apple-darwin9.8.0
>
> locale:
> [1] en_US.UTF-8
>
> attached base packages:
> [1] grid      stats     graphics  grDevices utils     datasets  methods
> [8] base
>
> other attached packages:
>  [1] HilbertVisGUI_1.3.3 HilbertVis_1.3.4    rtracklayer_1.5.17
>  [4] RCurl_1.2-0         bitops_1.0-4.1      ShortRead_1.3.36
>  [7] lattice_0.17-25     BSgenome_1.13.16    Biostrings_2.13.48
> [10] IRanges_1.3.87
>
> loaded via a namespace (and not attached):
> [1] Biobase_2.5.8 hwriter_1.1   tools_2.10.0  XML_2.6-0
> >
>
>
>
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>
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