Hi -
This is a recurring problem as more files are being deposited that contain only
references to other contigs but contain annotation and features.
A model where a DummySequence of appropriate length is instantiated if the contig
files are not available may be useful. Possibley a GBS parser would be useful? Has
anyone hacked out something like this?
- Mark
-----Original Message-----
From: [EMAIL PROTECTED] [mailto:[EMAIL PROTECTED]
Sent: Thu 11/09/2003 6:13 a.m.
To: biojava-l
Cc:
Subject: [Biojava-l] Parsing location from gbk files
Hi,
I am using biojava to extract mRNA, CDS and gene boundaries from genbank
annotation files (e.g., "hs_chr19.gbs" from
ftp://ftp.ncbi.nih.gov/genomes/H_sapiens/CHR_19/).
I've just switched from the biojava 1.22 to latest 1.3 release
(biojava-1.30-jdk14.jar and bytecode-0.92.jar)
and I get lots of exception (see below, the peace of code generating this
follows as well). Don't known if it's important but ".gbs" files do not hold the
contig sequences but only annotations.
Nota: I am using the jdk 1.4.1.
Thanks for your help.
Charles Girardot
=======================================
java.lang.IllegalArgumentException: Location 59817 is outside 1..0
at
org.biojava.bio.seq.impl.SimpleFeature.<init>(SimpleFeature.java:306)
at
org.biojava.bio.seq.impl.SimpleStrandedFeature.<init>(SimpleStrandedFeature.java:74)
at sun.reflect.GeneratedConstructorAccessor1.newInstance(Unknown
Source)
at
sun.reflect.DelegatingConstructorAccessorImpl.newInstance(DelegatingConstructorAccessorImpl.java:27)
at java.lang.reflect.Constructor.newInstance(Constructor.java:274)
at
org.biojava.bio.seq.SimpleFeatureRealizer$TemplateImpl.realize(SimpleFeatureRealizer.java:138)
rethrown as org.biojava.bio.BioException: Couldn't realize feature
at
org.biojava.bio.seq.SimpleFeatureRealizer$TemplateImpl.realize(SimpleFeatureRealizer.java:144)
at
org.biojava.bio.seq.SimpleFeatureRealizer.realizeFeature(SimpleFeatureRealizer.java:94)
at
org.biojava.bio.seq.impl.SimpleSequence.realizeFeature(SimpleSequence.java:198)
at
org.biojava.bio.seq.impl.SimpleSequence.createFeature(SimpleSequence.java:204)
at
org.biojava.bio.seq.io.SequenceBuilderBase.makeSequence(SequenceBuilderBase.java:168)
at
org.biojava.bio.seq.io.SmartSequenceBuilder.makeSequence(SmartSequenceBuilder.java:87)
at
org.biojava.bio.seq.io.SequenceBuilderFilter.makeSequence(SequenceBuilderFilter.java:98)
at
org.biojava.bio.seq.io.StreamReader.nextSequence(StreamReader.java:101)
at org.gc.test.GenbankParserTest.main(GenbankParserTest.java:34)
==========================================
package org.gc.test;
import java.io.BufferedReader;
import java.io.File;
import java.io.FileReader;
import org.biojava.bio.seq.Sequence;
import org.biojava.bio.seq.SequenceIterator;
import org.biojava.bio.seq.io.SeqIOTools;
/**
* @author unknown
*
* To change the template for this generated type comment go to
* Window>Preferences>Java>Code Generation>Code and Comments
*/
public class GenbankParserTest {
public static void main(String[] args) {
System.out.println("Main starts ...");
try {
File f = new File("D:\\Travail\\data\\Hs_chr19.gbs");
BufferedReader bf = new BufferedReader(new FileReader(f));
SequenceIterator it = SeqIOTools.readGenbank(bf);
while (it.hasNext()) {
System.out.println("getting next seq...");
Sequence s = it.nextSequence();
}
} catch (Exception e) {
System.out.println(e.getStackTrace());
}
System.out.println("End of main");
}
}
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