Greetings all, Please refer to image 1 : http://i40.tinypic.com/11rsk00.png image 2 (end of graph): http://i44.tinypic.com/2vt4iu8.png
As shown in image (1) , I draw the basecall sequences of AB1 file,by parsing the ACE file generated by Phrap.That is ,for upper part I do not read AB1 file.Only read values from ACE file.(values under 'RD' tag in ACE file.)In the example, two files are 'sampleA-S-R.AB1' and 'sampleA-S-F.AB1'.
For the bottom part- that is to draw Chromatogram, I use Biojavas 'ChromatogramGraphic' class.
I wanted to align these two parts- that is align 'sampleA-S-R.AB1' basecall sequence with 'sampleA-S-R.AB1' chromatogram..and so on...(like in ChromasPro).
But I noticed that basecall sequence of 'ChromatogramGraphic' is totally different than that of from ACE file. Refer to image (2) ; you can see the two sequences generated by same AB1 file is different in lenght also.
What could be the problem here? Any suggestions please thanks in advance. umanga _______________________________________________ Biojava-l mailing list - [email protected] http://lists.open-bio.org/mailman/listinfo/biojava-l
