Hi Bernd

For the UCSC you need to filter on types. see http://genome.ucsc.edu/FAQ/FAQdownloads.html#downloads there is a section called "Downloading data from the UCSC DAS server"

for DAS libraries you can see a tutorial here 
http://www.biodas.org/wiki/DASWorkshop2010#Day_2

the one you would be most interested in is the Dasobert tutorial (http://www.ebi.ac.uk/~rafael/dokuwiki/doku.php?id=das:courses:dasobert ) for DAS client creation, but there is a also a good javascript library as well called JSDas.

Any more info then don't hesitate to ask.

Jonathan.

On 28 Apr 2010, at 08:25, Bernd Jagla wrote:

Hi there,       

I am trying to retrieve information (features) from the UCSC genome browser
using the DAS interface.
I am looking at the org.biojava.bio.program.das sources. I can retrieve all
top level entry points with
DASSequenceDB(dbURL)
(Apperently the last entry from the return XML object gives a
[Fatal Error] :1:1: Content is not allowed in prolog.
Which I am ignoring...)

and also the DSN entries using:
DAS das = new DAS();
   das.addDasURL(new URL(dbURLString));
for(Iterator i = das.getReferenceServers().iterator(); i.hasNext(); )
{....

When I try to access features for a top level entry point, i.e. a reference sequence I have the impression that first all features for a given reference
sequence are being downloaded.

My questions:

How can I access only the features of a specific region? I guess in DAS
terms I want to specify the segment part of the URL
(http://genome.ucsc.edu/cgi-bin/das/hg17/features?segment=22:15000000,160000
00).

I would also like to get the list of available features. How can I achieve this? From a wireshark output I can see that this is being retrieved somehow
behind the scene. How can I access this information?

I am looking at TestDAS*.java; are there any other examples around that I
can use to learn from?

Thanks a lot for your kind support,

Best,

Bernd


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Jonathan Warren
Senior Developer and DAS coordinator
[email protected]
Ext: 2314
Telephone: 01223 492314








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