On Wed, May 29, 2013 at 6:42 PM, Daniel Asarnow <[email protected]> wrote: > And the third sentence from that article is: "during transcription, a DNA > sequence is read by an RNA polymerase, which produces a *complementary*, > antiparallel RNA strand." > > mRNA is complementary to the *non-coding* strand. If the original sequence > is coding, you just change T and U. If not, you must make a complement > containing U. > > The example sequence is clearly coding, thus the confusion. > > -da
Hi Christina and all, This has come up on the Biopython mailing list a few times too. Does the strand section in the Biopython tutorial help at all? http://biopython.org/DIST/docs/tutorial/Tutorial.html#htoc24 "Before talking about transcription, I want to try and clarify the strand issue. Consider the following (made up) stretch of double stranded DNA which encodes a short peptide: ... The actual biological transcription process works from the template strand, doing a reverse complement (TCAG -> CUGA) to give the mRNA. However, in Biopython and bioinformatics in general, we typically work directly with the coding strand because this means we can get the mRNA sequence just by switching T -> U." Regards, Peter _______________________________________________ Biojava-l mailing list - [email protected] http://lists.open-bio.org/mailman/listinfo/biojava-l
