Dear Kavya, we encountered this issue for a protein complex at 95 mg/mL involving Human Serum Albumin (HSA) and a designed protein binder (Alphabody) as described in Pannecoucke et al. 2021 Sci Adv 7 (13), DOI:10.1126/sciadv.abe1682. The breakthrough in that case came from crystallization experiments at different temperatures (14C, 21C and 37C), with 37C coming out as the clear winner.
best wishes Savvas —— Prof. Savvas Savvides VIB Center for Inflammation Research Ghent University, Dept. of Biochemistry & Microbiology Technologiepark 71, 9052 Ghent, Belgium Email: savvas.savvi...@ugent.be<mailto:savvas.savvi...@ugent.be> ; savvas.savvi...@irc.vib-ugent.be<mailto:savvas.savvi...@irc.vib-ugent.be> Phone: +32 (0)472 928 519 (mobile) ; +32 (0)9 331 36 60 (office) Web:https://www.irc.ugent.be/index.php/groups/savvides-unit On 5 Feb 2024, at 11:27, kavyashreem <kavyashr...@instem.res.in> wrote: Dear All, Has anyone worked on a protein which is highly soluble even at 80mg/ml? We have one such candidate, which does not precipitate even at 80mg/ml instead forms phase separated globules in crystallization plate, which eventually hardens over a period of 1 to 1.5 months (which is florescent under UV microscope.) We tried screening at different pH, but failed to get any hits. Since we got few conditions in which the phase separated globules solidified, we focused on them and expanded with 120mg/ml protein, still there were not visible precipitates except for the phase separation. This has been a challenging target so far. We have tried with different constructs, which unfortunately are not soluble! Does POMs help in such cases? Or do you have any other suggestion. Thank you Regards Kavya CONFIDENTIAL: This email and any files transmitted with it are confidential and intended solely for the use of the individual or group to whom they are addressed. If you have received this email in error, please notify the sender immediately and delete this e-mail from your system. It is NOT AUTHORISED to copy, forward, or in any way reveal the contents of this message to anyone. ________________________________ To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
