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Artem G. Evdokimov wrote:

On the other hand, people will readily share horror stories about how
proteins only would crystallize for them once the purity exceeded a
magical percentage, or once a specific pesky impurity was removed, a
magical purification step was added, etc. Therefore, it is best to go
with the protein and figure things out practically :)

Or how a trace impurity crystallized when the target protein didn't,
and the mistake was only realized after MR failed,
MIR succeeded, and the density improved to the point where enough
sequence could be read off to identify it by blast.

Ed

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