*** For details on how to be removed from this list visit the ***
*** CCP4 home page http://www.ccp4.ac.uk ***
Just figured out solution 2 is the crystallographic 2-fold (110 + 70 = 180).
Solution 3 looks too weak to be significant. I think I'm clutching at
straws.
Sorry about my previous e-mail.
From: "A Hibbert" <[EMAIL PROTECTED]>
Just figured out solution 2 is the crystallographic 2-fold.
To: [email protected]
Subject: [ccp4bb]: More RFCORR/PHASER Questions
Date: Thu, 17 Nov 2005 20:47:58 +0000
*** For details on how to be removed from this list visit the ***
*** CCP4 home page http://www.ccp4.ac.uk ***
Sorry about all these questions, but I have a few more:
1) For general self-education, I tried out the RFCORR/AMORE script in the
RFCORR documentation. From what I understand, the X-rotation function
shouldn't need a .tab file (from TABLING) as normalized SFs from the model
are already calculated (and a TABLING run isn't included in the example).
Furthermore, the GENERATE command isn't executed. However, AMORE complains
that I haven't included a .tab file. Does anyone know why?
2) Do you think it's more sensitive doing a LOCKED ROTATION in AMORE, then
doing a translational search in PHASER, or doing everything in PHASER and
then running RFCORR?
P.S: The crystal is P2(1) [64.0600 79.1400 71.0660 90.0000 110.9270
90.0000], with 2 molecules per ASU, and the following self-Patterson
solution(s) (quickly run in MOLREP):
#Sol_RF 1 0.00 0.00 0.00 270.00 0.00 90.00 3212.
26.18
Sol_RF 2 70.90 0.00 180.00 0.00 141.81 180.00 1238.
10.09
#Sol_RF 3 146.27 0.00 180.00 180.00 67.46 0.00 433.6
3.53
#Sol_RF 4 48.18 0.00 180.00 0.00 96.36 180.00 376.4
3.07
#Sol_RF 5 85.47 0.00 180.00 0.00 170.94 180.00 331.5
2.70
#Sol_RF 6 86.29 80.98 180.00 80.98 172.57 99.02 317.3
2.59
#Sol_RF 7 157.51 0.00 19.50 260.98 7.43 80.98 317.3
2.59
#Sol_RF 8 0.00 -45.00 170.44 170.44 0.00 0.00 294.4
2.40
#Sol_RF 9 0.00 45.00 -170.44 189.56 0.00 0.00 294.4
2.40
#Sol_RF 10 72.70 10.28 180.00 10.28 145.41 169.72 291.4
2.38
Thanks for the comments.
_________________________________________________________________
On the road to retirement? Check out MSN Life Events for advice on how to
get there! http://lifeevents.msn.com/category.aspx?cid=Retirement
_________________________________________________________________
FREE pop-up blocking with the new MSN Toolbar get it now!
http://toolbar.msn.click-url.com/go/onm00200415ave/direct/01/
