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Orly,
you might want to try and rescreen. I had
problems getting high resolution diffraction for my 3C protease crystals from
foot-and-mouth disease virus during my PhD. My initial crystals gave anisotropic
diffraction 3.5 angstrom at best in 1 direction and about 6 in the other. I
managed to solve the structure eventually by developing a novel ''trick'' for
improving the crystals (I got 1.9 angstrom in the end). It's very
easy!!
It involves mixing the original
crystallisation condition back into the sparse matrix screens. My experience was
that it RAPIDLY allowed me to find useful additives from the screens. My 1 year
of searching for additives ended with my mixing trick and I got high resolution
diffraction within 1 WEEK of trying.
Look at my paper Birtley and Curry Acta Crystallogr D Biol
Crystallogr. 2005
May;61(Pt 5):646-50. Epub 2005 Apr 20.
Good luck and let me know if it's as successful for you as it was
for me.
All the best.
Dr James Birtley From: [EMAIL PROTECTED] on behalf of Orly Dym Sent: Sun 27/11/2005 08:31 To: [email protected] Subject: [ccp4bb]: I have tried adding additives, crystallizing in different methods (hanging,
drops, sitting drops and microbatch under oil).
The crystals grow over night and contain high percent of water
(they are very fragile).
I also tried different methods of dehydration (using different PEG's, MPD,
glycerol and so on).
Currently I am trying to grow them at 4C.
Any other ideas as to what I should try in order to improve the
resolution?
Thanks
Orly
Dr. Orly Dym
Israel Structural Proteomics Center
Department of Structural Biology
Weizmann Institute of Science
http://www.weizmann.ac.il/ISPC 972-8-934-3759 - Tel
972-8-934-4159 - Fax
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