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Hello, do you include SDS-PAGE in "biochemical studies"? I would be very surprised if the cut was real and you still would see one 700aa band on a gel, so maybe there is just a break in the density? Tim -- Tim Gruene Institut fuer anorganische Chemie Tammannstr. 4 D-37077 Goettingen GPG Key ID = A46BEE1A On Thu, 1 Dec 2005, Jinkwang wrote: > Hi all, > > > > Routine MR solution of a mutant structure was an easy task until I found a > clear cleavage within the polypeptide chain. This cleavage is reproducible > only in crystal structure, and biochemical studies such as SDS-PAGE, > N-terminal sequencing or Mass spectra never indicate any cleavage in the > chain. > > > > The protein has about 700 amino acids, divided into alpha (200 a.a) and beta > (500) chains. The cleavage is found at 28-29 of the beta chain in the > crystal. Wondering whether this could occur only in crystal states, the > crystals were dissolved, but the biochemical studies did not show any > cleavage, but crystal structure once again shows. > > > > Any comments are highly appreciated. > > > > Regards, > > Jin Kwang > > > > > >
