Here is a summary:
X-ray crystal structures phased with EM envelopes include the pea light harvesting complex trimer (1), ClpP protease (2), tricorn protease (3), HK97 virus (4), reovirus (5), bluetongue virus (6), phiX174 procapsid (7,8), Norwalk virus (9), hepatitis B virus (10), 50S ribosomal subunits (11,12), and the 70S ribosome (13).
1. Standfuss, J., Terwisscha van Scheltinga, A. C., Lamborghini, M., and Kuhlbrandt, W. (2005) Embo J 24, 919-928
2. Wang, J., Hartling, J. A., and Flanagan, J. M. (1998) J Struct Biol 124, 151-163
3. Bosch, J., Tamura, T., Bourenkov, G., Baumeister, W., and Essen, L. O. (2001) J Struct Biol 134, 83-87
4. Helgstrand, C., Wikoff, W. R., Duda, R. L., Hendrix, R. W., Johnson, J. E., and Liljas, L. (2003) J Mol Biol 334, 885-899
5. Reinisch, K. M., Nibert, M. L., and Harrison, S. C. (2000) Nature 404, 960-967
6. Grimes, J. M., Burroughs, J. N., Gouet, P., Diprose, J. M., Malby, R., Zientara, S., Mertens, P. P., and Stuart, D. I. (1998) Nature 395, 470-478
7. Dokland, T., McKenna, R., Ilag, L. L., Bowman, B. R., Incardona, N. L., Fane, B. A., and Rossmann, M. G. (1997) Nature 389, 308-313
8. Dokland, T., McKenna, R., Sherman, D. M., Bowman, B. R., Bean, W. F., and Rossmann, M. G. (1998) Acta Crystallogr D Biol Crystallogr 54 ( Pt 5), 878-890
9. Prasad, B. V., Hardy, M. E., Dokland, T., Bella, J., Rossmann, M. G., and Estes, M. K. (1999) Science 286, 287-290
10. Wynne, S. A., Crowther, R. A., and Leslie, A. G. (1999) Mol Cell 3, 771-780
11. Ban, N., Freeborn, B., Nissen, P., Penczek, P., Grassucci, R. A., Sweet, R., Frank, J., Moore, P. B., Steitz, T. A. (1998) Cell 93, 1105-1115
12. Thygesen, J., Weinstein, S., Franceschi, F., and Yonath, A. (1996) Structure 4, 513-518
13. Cate, J. H., Yusupov, M. M., Yusupova, G. Z., Earnest, T. N., and Noller, H. F. (1999) Science 285, 2095-2104
-----Original Message-----
From: Cathy Lawson <[EMAIL PROTECTED]>
To: ccp4bb dl.ac.uk <[email protected]>
Sent: Mon, 28 Nov 2005 16:47:39 -0500
Subject: [ccp4bb]: examples needed of cryoEM maps used for phasing
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Dear CCP4 bb colleagues,
I'm seeking examples of X-ray crystal structures phased with cryoEM envelopes.
I am aware of two cases: 50S ribosome subunit (Ban et al 1998), HK97 virus (Helgstrand et al 2003).
Please reply to me directly if you know of others--I'll eventually post a summary.
thanks,
Cathy Lawson
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Catherine Lawson
Laboratory for Structural Biology and Bioinformatics
Department of Chemistry and Chemical Biology
610 Taylor Road
Piscataway, NJ 08854 USA
http://roma.rutgers.edu
http://rutchem.rutgers.edu/~lawson
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