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Dear All,
Sorry for the long silence.
The dip in anom correlation is a real effect and occurs at other light sources
(several people wrote to me) not just ID29. We have suggestion on how to solve
it. One is to change the tolerance keyword in MOSFLM. Another is to use XDS.
It is my intention to systematically evaluate these and summarise this on the
BB. We are going to send images to Harry P who has offered to have a look.
However, we delayed in doing this as we solved the structure using one from
several data sets.
This also had the dip but the correlation was in general much higher. The
structure could only be solved on something like ID29. SHELXD found a solution
on one peak data set at trial 150, found 80 Se atoms. On average 1 in 600 slns
(George Sheldrick ran it to exhaustion).
So to summarise, you can solve really hard things on ID29. There are however
issues in exactly the best way to process your data from these tiled arrays
where it is really really marginal (~3.0A). When the data is better the problem
is not fatal and is usually ignored.
As I have to go back and reprocess data which we don't need and did not solve
the structure and do comparisons, I will do it but not before Xmas. I might
treat myself to do some over the holidays.
Best
Jim
James H. Naismith | Research mailto:[EMAIL PROTECTED]
Professor of Chemical Biology | Teaching mailto:[EMAIL PROTECTED]
BBSRC Career Development Fellow |
Centre for Biomolecular Sciences | Office: 1334-463792 (24 hr)
The North Haugh | Fax : 1334-467229
The University | Lab : 1334-467245
St. Andrews | In UK add 0 to start of number
Fife Scotland, U.K., KY16 9ST | http://www.st-and.ac.uk/~strucbio
