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Deal All,

I'm posting this on behalf of prof Jerry Wells of UvA
([EMAIL PROTECTED]), please respond directly to him...
Its protein expression mainly, but the project has a structure-based
drive.

Flip
----------------------------
Post-doctoral Research Scientist and Analyst to work on Antimicrobial
Drug Discovery

3 year post-doctoral position and 1 year funding for an analyst
-starting Jan 1, 2006

Background to the Project 
The acquisition of multi-drug resistance has rendered some hospital
acquired infections and certain causes of community acquired pneumonia,
meningitis and sepsis impossible to treat. Unless strategies for the
development of alternative antibiotics are initiated soon, this will
have an enormous social impact on the sustainability of other
developments in modern medicine and quality of life worldwide. This
Senter funded project involves two pharmaceutical companies and one
other academic group with a combined aim of discovering new novel
antibiotics against the relentless rise of infection caused by
multi-drug (methicillin) resistant Staphylococcus aureus (MRSA),
multi-drug resistant Streptococcus pneumoniae and vancomycin resistant
Enterococcus (VRE). There are also clear possibilities to modify the
drugs developed in this project to have activity against other kinds of
drug resistant bacteria (e.g. see Current Opinion in Microbiology 2004,
7:439-444).
Our strategy focuses on blocking the activities of an essential
two-component regulatory system YycFG which is crucial for signaling in
the disease target organisms (e.g. see Mohedano et al., J Bacteriol.
2005, vol 187:2357-67 & Stephenson et al., Current Opinion in
pharmacology 2002, vol 2:1-6). The role of The University of Amsterdam
in this project is to investigate the role of this two-component system
and the mechanism of regulation in detail e.g. using DNA binding assays,
quantitative PCR, regulatory mutants and genetic reporter systems.
Additionally we will participate in determining the target protein
structures and drug modeling through the expression of soluble proteins
or protein domains and biochemical assay development. 

Further Information 
Ideally the post-doc will be a molecular biologist with previous
experience of expressing and purifying proteins, microarray studies and
of working with Gram-positive bacteria. Applications are also welcome
from persons with basic knowledge in one or more of these areas who wish
to develop new skills. The vacancies exist within the Cellular
Microbiology Research Group headed by Prof. Jerry Wells This research
group resides within the Swammerdam Institute for Life Sciences (SILS)
the UVA Science Faculty's largest institute and shares excellent
laboratory facilities and equipment with other microbiology groups
working on topics such as gene regulation, fermentation, sporulation,
host microbe interactions and systems biology.
For further information on how to apply for these posts please contact
[EMAIL PROTECTED] or visit the UVA job vacancies web site:
http://www.english.uva.nl/news/uva_vacancies.cfm


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