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Hi Tiago, Properties of (growing) lysozyme crystals have been studied with EM and Atomic Force Microscopy. I did not bother to look up any refs, but that should be easy... Flip -----Original Message----- From: [EMAIL PROTECTED] [mailto:[EMAIL PROTECTED] On Behalf Of Tiago Barros Sent: Monday, 12 December, 2005 17:37 To: [email protected] Subject: [ccp4bb]: Analyse crystals without X-rays *** For details on how to be removed from this list visit the *** *** CCP4 home page http://www.ccp4.ac.uk *** Dear all, Apologies for a non-ccp4 subject. I've been assigned to give a small talk in our "X-ray meeting" here at the lab. I thought it would be interesting to discuss how to analyse protein crystals without X-rays. Obviously our ultimate goal is a diffraction pattern good enough to solve our structure, but in many cases other valuable information could be obtained using other, complementary techniques. Working with a colourful protein, multiple spectroscopic methods (absorption, fluorescence, etc) popped up in my mind as examples of those techniques. Chromatographic methods (HPLC?) for chemical analysis also look likely candidates. What else could you add to the discussion? Does anyone have personal experience on "How to analyse crystals without X-rays"? Thank you very much for any suggestions. Tiago ------------------------------------------------------------ Tiago Barros Max-Planck Institute of Biophysics Dept. of Structural Biology Max-von-Laue Str. 3 D-60438 Frankfurt Germany tel: +49-69-6303-3055
