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To avoid that people start to think high solvent content is actually
desirable, the honking big yellow crystal that Emerald uses on their
promotional pictures had about 85% solvent content. Although crystals
grew up to 2x2x1.5mm diffraction never exceeded 9 Angstrom. I should
mention that this was before routine cryo-mounting and we were dealing
with a 9 MDa protein and unit cell axes close to or exceeding 1000
Angstrom along each axis. So it was a bit of a long shot to start with.
Bart
Michael Hadders wrote:
Another example: beta 2 glycoprotein 1 [Bouma et al. (1999) EMBO J.
18:5166-5174]. Crystals had solvent content of 86% (Vm=8.5) and
diffracted to 2.7 angs. resolution.
Cheers,
Michael
--
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Bart Hazes (Assistant Professor)
Dept. of Medical Microbiology & Immunology
University of Alberta
1-15 Medical Sciences Building
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Canada, T6G 2H7
phone: 1-780-492-0042
fax: 1-780-492-7521
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