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The Fourth International Workshop on X-ray Damage to Biological
Crystalline Samples will be held on March 7th and 8th, at SPring8, Japan.
WWW site for registration and accommodation (closes 31st Jan 2006)
http://www.spring8.or.jp/e/conference/rd4/
Organisers: Soichi Wakatsuki, Masaki Yamamoto, Elspeth Garman,
Colin Nave, Sean McSweeney, Raimond Ravelli and Gerd Rosenbaum.
Programme:
(a) Experience from electron microscopy.
(i) Bill Massover, New Jersey Medical School, U.S.A.
Radiation damage to protein specimens from modern electron microscope
imaging and diffraction: a Minireview.
(ii) Yoshimasa Fujiyoshi, Kyoto University, Japan
Cryo-protection and Cryo-electron microscopy
(iii) Ishikawa Tetuya, RIKEN SPring-8 Center, Japan. tba
(b) Relevant radiation chemistry.
(i) Ian Carmichael, Notre Dame Radiation Laboratory, Indiana, U.S.A.
Radiation chemistry of aqueous amino acids
(ii) Andrea Schmidt, EMBL, Hamburg, Germany.
How to avoid premature decay of your macromolecular crystal - a
chemist's approach to combat physical reality.
(c) Beam heating; modelling and verification.
(i) Michael Kazmierczak, University of Cincinnati, U.S.A.
Thermal Imaging Applied to Cryocrystallography: Cryocooling and Beam
Heating (Pt I)
(ii) Eddie Snell, Hauptmann Woodward Institute, Buffalo, U.S.A.
Thermal Imaging Applied to Cryocrystallography: Beam Heating of Samples:
Modeling and Verification (Pt II)
(d) Dose/dose rate effects and limits to sample life.
(i) Gleb Bourenkov, MPI, Hamburg, Germany.
Modeling the intensity variation with the X-ray dose
(ii) Shimizu Nobutaka and Yamamoto Masaki
Radiation Damage of Protein Crystal in various X-ray energies
(iii) Colin Nave, SRS, small crystals. U.K
What is the Optimum Wavelength to Study a Protein Crystal of a Given Size?
(iv) Armin Wagner, DLS, Didcot, U.K.
Systematic Study on the Effect of Cryocooling of Protein Crystals to 10K.
(v) Elspeth Garman, University of Oxford, U.K.
Experimental determination of the radiation dose limit for cryo-cooled
protein crystals.
(e) Specific sensitivity of particular crystals/heavy atoms to damage,
(i) Raimond Ravelli, EMBL, Grenoble, France
Specific Radiation Damage versus Global Non-Isomorphism
(ii) James Holton, ALS, Berkeley, U.S.A.
A fluorescence probe for site specific damage.
(iii) Udupi Ramagopal, BNL, U.S.A.
Radiation-induced site-specific damage of mercury derivatives: phasing
and implications.
(f) Improved software for dealing with radiation damage:
(i) Sasha Popov, EMBL, Hamburg, Germany
Handling the radiation damage in BEST
(ii) Marc Schiltz, Lausanne University, Switzerland.
Detection and modeling of site-specific radiation damage in SAD/MAD
experiments.
(iii) Gerard Bricogne, Global Phasing, Cambridge, U.K. tba
(iv) Zbyszek Otwinowski, UT South Western Medical Center Dallas, U.S.A.
Complex Impact of Radiation Damage on Phasing
(g) The utilisation of radiation damage for biological studies.
(i) Tsutomu Kouyama, Nagoya University, Japan. tba
(ii) Kristina Djinovic-Carugo, University of Vienna, Austria
Reduction of metal centres by X-rays: case studies
(iii) Martin Weik, IBS, Grenoble, France
Specific radiation damage to acidic residues in protein crystals
(iv) Remco Kort, University of Amsterdam, Holland
Biological implications of radiation damage in the active site:
Cryocrystallographic studies on DNA photolyase and photoactive yellow
protein.
(v) Ilme Schlichting, MPI, Heidelberg, Germany.
X-ray crystallographic studies of redox-sensitive proteins
(h) Imaging and Radiation Biology of whole cell.
(i) Sean McSweeney, ESRF
Exploring an ionising radiation resistant phenotype.
(ii) Nishino Yoshinor, RIKEN SPring-8 Center, Japan.
High-Spatial-Resolution X-ray Diffraction Microscopy
(i) Chris Jacobsen, Stony Brook University, U.S.A.
Radiation damage at different scales: from molecules to cells
------------------------------------------------------------------
Dr. Elspeth F. Garman,
Reader in Molecular Biophysics,
Department of Biochemistry,
Rex Richards Building,
University of Oxford, Tel: (44)-1865-275398
South Parks Road, FAX: (44)-1865-275182
OXFORD, OX1 3QU, U.K. E-mail: [EMAIL PROTECTED]
