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I am very much convinced with Dr. Carlos experience, same I too experienced.
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Stefano Benini wrote:
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Dear crystallisators,
I would like to know what people usually tries (possibly succesfully!) to
get not twinned crystals when a protein tends to crystallise as a perfect
twin
I am trying/plannning to try: finding new conditions, additives,
detergents, tag removed, new construct, etc.,
Hi
In one case I succeeded to get non observable twinning by changing the pH
on 0.5 units, on another case by changing the crystallization from room
temperature (say 20 C) to that in the cold room (say 4-10 C).
Lots of good luck!
Carlos
Thank you very much
Ciao
Stefano
**********************************
Stefano Benini Ph.D.
http://www.ysbl.york.ac.uk/~benini
York Structural Biology Laboratory
University of York
Heslington York YO10 5YW United Kingdom
Tel.:+44 1904 328276
Fax: +44 1904 328266
"verba volant scripta manent"
**********************************
--
**************************************
Dr. Carlos Frazao
Crystallography Department
ITQB-UNL, Av Republica, Apartado 127
2781-901 Oeiras, Portugal
Phone: (351)-214469666 FAX: (351)-214433644
e-mail: [EMAIL PROTECTED]
www.itqb.unl.pt
