The analysis Ray Withers sent is available at the following link.

http://sbl.unmc.edu/files/geob_diff_pattern.pdf


----- Forwarded by Gloria Borgstahl/Eppley/UNMC/UNEBR on 02/08/2006 12:44 PM -----
Tommi Kajander <[EMAIL PROTECTED]>

02/06/2006 12:56 PM

To
Gloria Borgstahl <[EMAIL PROTECTED]>
cc
Subject
Re: Fw: [ccp4bb]: Curious diffraction pattern - non CCP4 related





Hi, would you mind putting the pdf on web or sending me a copy? I'd be
interested. --the mailing lists dont allow attachements.
thanks a lot,
tommi

Quoting Gloria Borgstahl <[EMAIL PROTECTED]>:

> Dear Tiago,
>
> I asked a few colleagues who are experts in aperiodic and quasi crystals.
> Ray Withers replied in the email below.
> I converted the ppt file he refers to as a pdf, so it is not so large.
> Take a look at his analysis there.
> Very interesting, so maybe there are more modulated protein crystals out
> there than we thought.
> I also asked Christer Svensson what he thought  and he said "It might of
> course be something complicated, like a modulation.
> But looking at just the first of the pictures, this pattern could be
> generated by "twinning" of  a hexagonal lattice.
> One part of the crystal is rotated (around the 3 or 6-fold axis) about 22
> degrees with respect to the other.
> It would be a lot easier to judge by doing the indexing."
>
> Christer's program TwinSolve can handle this type of data, you may want to
> contact him.
>
> Have fun with it. Gloria
>
> ----- Forwarded by Gloria Borgstahl/Eppley/UNMC/UNEBR on 02/05/2006 08:01
> PM -----
>
> [EMAIL PROTECTED]
> 02/03/2006 04:55 PM
>
> To
> "Gloria Borgstahl" <[EMAIL PROTECTED]>
> cc
>
> Subject
> Re: Fw: [ccp4bb]: Curious diffraction pattern - non CCP4 related
>
>
>
>
>
>
> G'day Gloria,
>
> Very interesting. Looks very much like a so-called triple-q modulated
> structure (see the attached .ppt file. This sort of thing sometimes occurs
> as a Moire layer stacking effect.
>
>
> With best wishes,
>
>
> Ray
>
>
> Hi Ray, just received your aperiodic email and then this one off the ccp4
>> group.
>> What do you make of this diffraction?  Gloria
>>
>> ----- Forwarded by Gloria Borgstahl/Eppley/UNMC/UNEBR on 02/03/2006
> 09:18
>> AM -----
>>
>> Tiago Barros <[EMAIL PROTECTED]>
>> Sent by: [EMAIL PROTECTED]
>> 02/03/2006 08:59 AM
>>
>> To
>> [email protected]
>> cc
>>
>> Subject
>> [ccp4bb]: Curious diffraction pattern - non CCP4 related
>>
>>
>>
>>
>>
>>
>> ***  For details on how to be removed from this list visit the  ***
>> ***          CCP4 home page http://www.ccp4.ac.uk         ***
>>
>>
>> Dear all,
>>
>> I would like to share with you a very strange diffraction pattern
>> that I recently collected at the SLS.
>>
>> You can have a look at some pictures of this diffraction pattern here:
>>
>> http://tiagobarros.home.sapo.pt/zoom_in.png
>>
>> http://tiagobarros.home.sapo.pt/45.png (45 degrees)
>>
>> http://tiagobarros.home.sapo.pt/90.png
>>
>> http://tiagobarros.home.sapo.pt/135.png
>>
>> Some additional information. These happened with just one of my
>> crystals. I know from previous experience that my crystals (from a
>> membrane protein) are made of stacks of 2D crystals (SG P321). This
>> stacking is very often not that ordered, which leads to different
>> layers with different orientation within the same crystal. The space
>> group of my crystals is usually either C2 or P3121.
>>
>> I've found these circular motif of spots very curious, and I don't
>> really have a good explanation for them.
>>
>> Any idea of what might have caused this?
>>
>> Thank you.
>>
>> Tiago
>>
>>
>> ************************************************************************
>> *
>>
>> Tiago Barros
>> Max-Planck Institute of Biophysics
>> Dept. of Structural Biology
>> Max-von-Laue Str. 3
>> D-60438 Frankfurt
>> Germany
>>
>> tel:  +49-69-6303-3055
>>
>> ************************************************************************
>> *
>>
>>
>>
>>
>>
> [attachment "Gloria.ppt" deleted by Gloria Borgstahl/Eppley/UNMC/UNEBR]



------------------------------------------
Tommi Kajander, Ph.D.
Post Doc Fellow
Molecular Biophysics and Biochemistry
Yale University
PO Box 208114
New Haven, CT 06520-8114, USA
http://www.csb.yale.edu/people/regan/people.html
p. +1-203-432 9841
fax +1-203-432 5175

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