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We are expressing a cell adhesion protein which is very
heterogeneous in it's pI in Pichia. After Deglycosylation by Endo H or by Endo F1 we can see 3 bands. Can anyone suggest what is the reason for this heterogeneity?



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Dr. Michal Harel
Dept. of Structural Biology          [EMAIL PROTECTED]
Weizmann Inst. of Science                  tel:  972-8-9342647
Rehovot 76100, ISRAEL                      fax:  972-8-9344159
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