[ccp4bb]: good rotation, not so good translation

[Angela Hoffort]

I am in working on data processed to 3.3A in H3. Using Phaser and a tetramer model created from running a monomer through Molrep, I get pretty good rotation solutions (based on Z-score). However, the problem occurs when these solutions are run through the fast translation portion of Phaser. After translation there appears to be no real solutin and the Z-scores decrease considerably. Can someone help explain why this may be happening and perhaps suggest a different method of translation. Below is a list of the translation solutions. Note the decrease from RFZ to TFZ.


fast translation H3 with tetramer from molrep
SPAC R 3
SOLU SET  RFZ=19.6 TFZ=4.4
SOLU 6DIM ENSE tetra EULER   99.104    0.450  140.458 FRAC  0.78046  0.16014  0.00000
SOLU SET  RFZ=19.6 TFZ=4.0
SOLU 6DIM ENSE tetra EULER   99.104    0.450  140.458 FRAC  0.80130  0.16014  0.00000
SOLU SET  RFZ=19.2 TFZ=4.2
SOLU 6DIM ENSE tetra EULER   90.362  108.895  330.184 FRAC  0.64129  0.56287  0.00000
SOLU SET  RFZ=19.2 TFZ=4.2
SOLU 6DIM ENSE tetra EULER   90.362  108.895  330.184 FRAC  0.74962  0.46704  0.00000
SOLU SET  RFZ=19.6 TFZ=3.4
SOLU 6DIM ENSE tetra EULER   99.104    0.450  140.458 FRAC  0.82213  0.24348  0.00000
SOLU SET  RFZ=19.2 TFZ=3.7
SOLU 6DIM ENSE tetra EULER   90.362  108.895  330.184 FRAC  0.69129  0.66704  0.00000
SOLU SET  RFZ=19.2 TFZ=3.7
SOLU 6DIM ENSE tetra EULER   90.362  108.895  330.184 FRAC  0.67879  0.03370  0.00000
SOLU SET  RFZ=19.2 TFZ=3.6
SOLU 6DIM ENSE tetra EULER   90.362  108.895  330.184 FRAC  0.64129  0.76287  0.00000

Thank you,
Angela Hoffort