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Dear CCP4ers,
Nat Echols wrote:
...
I would definitely say that reproducing the crystals in a more standard
format (i.e. 24-well sitting/hanging drop trays) doesn't always work as
expected, but I've had this problem regardless of screen format. (I
suspect as more and more people use robots this will continue to be an
issue.)
...
yes, we have quite often problems now with upscaling an initial
crystallization condition from our robot 96 well plates with typically
200-500 + 200-500 nl drop size to conventional 24 wells with 2-3 + 2-3
ul drop size. It's clear, that the geometry is somewhat different, and
presumably the smaller drops mix faster, but currently, I have no idea
what the major obstacle for upscaling is. Does anybody of you have a
clue and, maybe, a solution or workaround?
Best regards,
Dirk.
--
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Dirk Kostrewa
Paul Scherrer Institut
Biomolecular Research, OFLC/110
CH-5232 Villigen PSI, Switzerland
Phone: +41-56-310-4722
Fax: +41-56-310-5288
E-mail: [EMAIL PROTECTED]
http://sb.web.psi.ch
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