RE: RE: [ccp4bb]: Paper Help--looking for crystals grown for a long time and with few waters

[Artem Evdokimov]

Hi,   With the parameters you indicated, you have two likely possibilities �C either a monomer with 70% solvent content, or a dimer with 40% solvent content. A trimer would mean 13% solvent content �C way too low to be likely.   For a 2.5 A structure, finding 26 well-defined waters is more or less OK, even if you have a large-ish unit cell. I doubt that this has much to do with the age of the crystal �C after all, the crystal is still either 70 or 50 % solvent. At this resolution, any kind of bulk solvent correction is likely to obscure many of the exterior solvent molecules.   Artem   From: [EMAIL PROTECTED] [mailto:[EMAIL PROTECTED] On Behalf Of LiangZhang Sent: Tuesday, June 06, 2006 12:51 AM To: ccp4bb Subject: Re: RE: [ccp4bb]: Paper Help--looking for crystals grown for a long time and with few waters   Thanks for your reply.   The resolution is 2.5 A. The crystal is P1211, a=40,  b=75,   c=99;  α=90,  β=101,  γ =90      The reservoir solution contains PEG10000 and MPD.     LiangZhang 2006-06-06 发件人： Artem Evdokimov 发送时间： 2006-06-06 12:37:32 收件人： 'LiangZhang'; 'ccp4bb' 抄送： 主题： RE: [ccp4bb]: Paper Help--looking for crystals grown for a long time and with few waters   What’s the resolution and VM of your crystal? It’s very unlikely that you have only 26 water molecules around �C but you may not be able to locate most of the solvent present, if the resolution is too low and/or the disorder too high…   Artem   From: [EMAIL PROTECTED] [mailto:[EMAIL PROTECTED] On Behalf Of LiangZhang Sent: Tuesday, June 06, 2006 12:00 AM To: ccp4bb Subject: [ccp4bb]: Paper Help--looking for crystals grown for a long time and with few waters   Hi all,        Sorry for the non-ccp4 topic.        I have a protein crystal(310aa) grown for one year and collected data from it. After structure determination we found only 26 water molecules in one monomer.  I hope to find some papers which described crystals grown for a long time and with few water molecules. Could any one help me find some?        Thanks for you help!     LiangZhang 2006-06-06