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Sorry for the off-topic question!
 
My current construct of a new protein (has some homologs) doesn't seem to be 
good and I have to make some new ones. I am just wondering how to determine the 
N-terminus & C-terminus of new constructs if I am only interested in structural 
study of a few domains. Any good methods or softwares to recommend?  Thanks a 
lot! 
 
Yeming
---------------------
Yeming Wang, Ph.D. 
Laboratory of Structural Biology: Macromolecular Structure Group 
National Institute of Environmental Health Sciences 
National Institute of Health 
Mailing Address:      Street Address: 
NIEHS, MD F3-05       NIEHS, Building 101, Room F362 
P.O. BOX 12233        111 T.W. Alexander Drive 
RTP, NC 27709         RTP, NC 27709 
Tel (o): 919-316-4634 
E-mail: [EMAIL PROTECTED]

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