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Hi,
I can suggest 2 options:
1) Use CAD and SIGMAA from CCP4 to combine the HL coefficients from the
experimental phases at 2.1A with the F and SIGF from the native to
1.95A. In RESOLVE, provide this combined data set and the partial model
as input, set resolution limit to 1.95A and use resolve_build mode. This
will allow density modification and phase extension to 1.95A and will
allow further automatic rebuilding of the model to 1.95A. Actually, you
can do this without providing the input model. RESOLVE will
automatically try to build a new model from the new maps with extended
phases.
2) Use the parital model to do molecular replacement with the native
data set and then refine the molecular replacement solution using the
full data to 1.95A.
Good luck!
Debanu.
--
Debanu Das,
Berkeley.
Mario Sanches wrote:
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Dear colleagues,
I just solved a structure by SAD using a soaked crystal with NaI, on a
2.1A dataset, spacegroup C2. Using this dataset I partially
constructed a model. Now I would like to extend the phases to a native
dataset which was processed at 1.95A resolution, spacegroup also C2.
Which is the correct way to do this and still be able to use my
partially constructed model?
Thank you all in advance,
Mario Sanches
