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-----BEGIN PGP SIGNED MESSAGE----- Hash: SHA1 Hi Neil, It may depend a lot on your expression system, buffers throughout the purification/crystallization, etc. My guess it that it looks like N-terminal acetylation. If your protein is not too big you should be able to see this by ESI-MS. Cheers, Miguel En/na Neil Paterson ha escrit: > Dear all, > > I'm currently puzzled by some N-terminal density visible in a 1.4 > angstrom map (image <http://www.chem.gla.ac.uk/%7Eneison/blob.png>). My > protein has been cleaved with 3C protease, cleavage confirmed by > SDS-PAGE and failure to rebind a NiNTA column. 3C should cleave > LEVLFQ/GP yet there appears to be strong density preceding the glycine. > The density is not a great fit for an additional residue (although there > shouldn't be one) and I'm sure my glycine is in the correct place as the > proline density is very clear. Has anyone seen anything like this before > or have any suggestions as to what it may be (and how to model it)? > > Thanks, > Neil - -- Miguel Ortiz Lombardía Centro de Investigaciones Oncológicas C/ Melchor Fernández Almagro, 3 28029 Madrid, Spain Tel. +34 912 246 900 Fax. +34 912 246 976 e-mail: [EMAIL PROTECTED] - ---------------------------------------------------------------------- Et ainsi ne pouvant faire que ce qui est juste fût fort, on a fait que ce qui est fort fût juste. Blaise Pascal, Pensées -----BEGIN PGP SIGNATURE----- Version: GnuPG v1.4.1 (Darwin) iD8DBQFEmtuZF6oOrDvhbQIRAu9EAJ4oDu8gIf/WO/ycspk5FHBZXHsSKwCdEZoz xF0VmJLFoHQx3gjpIA6w17M= =O7Nt -----END PGP SIGNATURE-----
