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For phasing purposes: very careful scaling of the data, e.g. scala. Does
not matter if your completeness goes down. Even from (say) 70% to 35% in
the high resolution bin when going from automagic to very careful scaling.
For structure determination and refinement (once experimental phases have
been determined): getting a complete data set is really important, thus
automated scaling programs or procedures (e.g. xscale from Kabsch's XDS
package) will do fine. This is because holes in the diffraction pattern
leads to effects in the FT of the diffraction pattern. Hence better to
have as few holes in the diffraction amplitudes as possible.
Fred.
--
s-mail: F.M.D. Vellieux (B.Sc., Ph.D.)
Institut de Biologie Structurale J.-P. Ebel CEA CNRS UJF
41 rue Jules Horowitz
38027 Grenoble Cedex 01
France
Tel: (+33) (0) 438789605
Fax: (+33) (0) 438785494
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