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Hi Y'all,
Non-xtallo query.
I have the foll. plasmids:
1) a pET21a vector (AmpR) expressing a single protein
2) a pET28 vector (KanR) containing a polycistronic construct that
co-expresses four proteins
While we contemplate sticking the fifth protein and making a single
multi-expression pET28 vector, I tried to co-transform the two plasmids
by bumping up the two antibiotics and that did not work.
If you have had success with co-transforming two plasmids that share the
same replication origin (for e.g., two different pET vectors) into
E.coli expression strains, could you pl. share your tricks and/or
suggestions. Do you have favourite strains/vectors when it works?
Many thanks.
Raji
--
Raji Edayathumangalam
Postdoctoral Associate
The Rockefeller University
Box 224. 1230 York Avenue
New York, NY 10021
