Re: [ccp4bb]: Off topic: E. coli soluble,small, monomeric basic protein

[Ashley Buckle]

Hi Rebecca You could use our REFOLD database for this. You can search with all your criteria in the advanced search. I found 3 hits, but this is of course limited by the size of our current database http://refold.med.monash.edu.au/search.php cheers ashley On 04/07/2006, at 1:14 AM, Page, Rebecca wrote: ***  For details on how to be removed from this list visit the  *** ***          CCP4 home page http://www.ccp4.ac.uk         *** Sorry for the off-topic question: I am in search of a protein with the following characteristics --small (< 15kD) --monomeric --highly soluble when expressed in E. coli  --basic pI (9.0 or higher). --preferably with the structure determined by x-ray crystallography, though not required (I haven't figured out a way to easily search for crystal structures using pI as a criteria in the RCSB, which might also provide some potential proteins with the above listed characteristics). Any advice/thoughts would be greatly appreciated! Thanks, Rebecca email: [EMAIL PROTECTED] Ashley Buckle Ph.D Senior Research Fellow The Department of Biochemistry and Molecular Biology School of Biomedical Sciences, Faculty of Medicine & Victorian Bioinformatics Consortium (VBC) Monash University, Clayton, Vic 3800 Australia http://www.med.monash.edu.au/biochem/staff/abuckle.html iChat/AIM: blindcaptaincat Tel: (613) 9905 3781 (office) Tel: (613) 9905 1653 (lab) Fax : (613) 9905 4699