[ccp4bb]: Phaser problem with high Z score

[OZ]

Dear ALL:   My data had a high Z-score(>10) with A template, but not output pdb. I check it out twice, it still not output pdb file.   With the same conditions, another Z-score(<10) was obtained with B template. And this time, we had a output pdb file. However, the score from B template was small than the one from A template. It confused me a lot. Any one had some appropriate explanation about that? Thank you very much.     jaishin             The .sol are described as below: A.template --------------- FINAL SELECTION ---------------      Mean (Sigma) used for final selection = -156.312 (24.0562)    Number of solutions stored before final selection = 4    Number of solutions stored (deleted) after final selection = 4 (0)      Fast Translation Function Table: Space Group R 3    ------------------------------------------------    #SET #TRIAL      Top    (Z)    Second    (Z)     Third    (Z)    Ensemble       1      1   192.15 (14.77)        -      -         -      -    ensemble1       1      2   160.66 (13.95)        -      -         -      -    ensemble1       1      3   160.29 (11.75)        -      -         -      -    ensemble1       1      4   148.37 (13.35)        -      -         -      -    ensemble1    ---- ------   ************************************************************************************* *** Phaser Module: MOLECULAR REPLACEMENT PACKING                            1.3.2 *** *************************************************************************************      Ensemble: ensemble1    -------------------    Structure with lowest rms = xxxx.pdb (1.5)    Trace length before selecting conserved = 763    Trace length after selecting conserved =  763      Solutions rejected if number of clashes > 0    #   #CLASHES     #   ACCEPTED       1   2                NO             2   2                NO             3   2                NO             4   2                NO               0 accepted of 4 solutions ################################################################################################## B.template: --------------- FINAL SELECTION ---------------      Mean (Sigma) used for final selection = -11.0591 (8.95763)    Number of solutions stored before final selection = 3    Number of solutions stored (deleted) after final selection = 1 (2)      Fast Translation Function Table: Space Group R 3    ------------------------------------------------    #SET #TRIAL      Top    (Z)    Second    (Z)     Third    (Z)    Ensemble       1      1    77.48 ( 9.52)        -      -         -      -    ensemble1       1      2    49.54 ( 6.77)        -      -         -      -    ensemble1       1      3    45.14 ( 6.69)        -      -         -      -    ensemble1    ---- ------   ************************************************************************************* *** Phaser Module: MOLECULAR REPLACEMENT PACKING                            1.3.2 *** *************************************************************************************      Ensemble: ensemble1    -------------------    Structure with lowest rms = .xxxxpdb (1.5)    Trace length before selecting conserved = 338    Trace length after selecting conserved =  338      Solutions rejected if number of clashes > 0    #   #CLASHES     #   ACCEPTED       1   0            1   YES              1 accepted of 1 solutions   ************************************************************************************* *** Phaser Module: MOLECULAR REPLACEMENT REFINEMENT AND PHASING             1.3.2 *** *************************************************************************************      Space-Group Name (Number):   R 3 (146)    Resolution of All Data (Number):       2.40 29.91 (35489)    Resolution of Selected Data (Number):  2.50 29.91 (31379)      Refinement Table: Space Group R 3    ---------------------------------    #+ = input number    #* = output number    Unsorted (refinement order)       Sorted in LL-gain order             Initial   Refined                     Initial   Refined     #+  LL-gain   LL-gain   Unique    #*  #+  LL-gain   LL-gain   Unique  =#*  =#+    1   77.48     88.24     YES       1   1   77.48     88.24     YES                   Mean used for final selection = -11.0591    Sigma used for final selection = 8.95763    Number of solutions stored before final selection = 1    Number of solutions stored (deleted) after final selection = 1 (0)   ************************************************************************************* *** Phaser Module: PERMUTATIONS AND SPACEGROUPS                             1.3.2 *** *************************************************************************************      NEW BEST SOLUTION: TOP LL-GAIN 88.236222    PERMUTATION #1    SEARCH ENSEMBLE ensemble1    SPACE GROUP: R 3   ************************************************************************************* *** Phaser Module: MOLECULAR REPLACEMENT REFINEMENT AND PHASING             1.3.2 *** *************************************************************************************      Space-Group Name (Number):   R 3 (146)    Resolution of All Data (Number):       2.40 29.91 (35489)    Resolution of Selected Data (Number):  2.40 29.91 (35489)      Refinement Table: Space Group R 3    ---------------------------------    #+ = input number    #* = output number    Unsorted (refinement order)       Sorted in LL-gain order             Initial   Refined                     Initial   Refined     #+  LL-gain   LL-gain   Unique    #*  #+  LL-gain   LL-gain   Unique  =#*  =#+    1   88.58     88.58     YES       1   1   88.58     88.58     YES                   Mean and Standard deviation for final selection not set    No final selection   ------------ OUTPUT FILES ------------      0803_3.sol    0803_3.1.pdb    0803_3.1.mtz