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Dear Yi,
I assume you searched with the protein alone. Look at the density. If
you see the DNA the solution is correct.
Best, Markus
Yi Xue schrieb:
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Dear all:
I have one dataset, diffracted to 3.2A.
It should be a protein-DNA complex. The protein structure has already
been solved. The DNA is a 23mer.
I used phaser to do the molecular replacement, and it found a solution,
RFZ=7.2, TFZ=11.9, however, LLG is negative.
I used the solution, did 20 cycles of restrained refinement, using
refmac.
R decreased from 0.476 to 0.354
Rfree decreased from 0.453 to 0.45.
Since there is only trivial improvement for Rfree, so is it possibly a
right solution?
thanks
Yi
