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There's a nice article about RNA crystallization: Golden, Kundrot (2003) Journal of Structural Biology 142, 98-107. In a fast scan, I don't see mention of citrate ion. It's amazing what I find in these piles around my desk. -Dan On Wed, 16 Aug 2006, William Scott wrote: > *** For details on how to be removed from this list visit the *** > *** CCP4 home page http://www.ccp4.ac.uk *** > > > > It is fairly common to get RNA (or DNA) crystals in high concentrations of > monovalent cations, including Na+, Li+, and even NH4+. More surprising to > us was that several of the ribozymes function quite happily in the > presence of monovalent cations that were thought to require divalent > cations for cleaving. > > One thing you will want to be aware of is that simple RNA hairpins have a > high propensity to crystallize as dimeric duplexes with mismatches in the > middle corresponding to the hairpin region. > > The anion is usually not relevant, but in the case of citrate, it can > chelate Mg++. For that reason, I tend not to use it in screens, but there > is no particularly fundamental reason why citrate should be unusual, apart > from that. > > HTH, > > Bill > > > > On Wed, 16 Aug 2006 [EMAIL PROTECTED] wrote: > > > Hi folks, > > > > I appear to have grown some crystals of an RNA hairpin using 1.4 M sodium > > citrate. > > > > Has anybody else out there been able to get crystals of RNA or DNA using > > sodium citrate as the precipitant ? > > > > This result seems to be rather unusual to me. > > > > Please get in touch if you know of any similar, successful crystallization > > conditions. > > > > > > Cheers, > > > > Ray > > > > email [EMAIL PROTECTED] > > > > >
