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Dear All,

I am on the final stage of refinement and I am making sure, on coot, that the residues are in the allowed regions of the Ramachandran Plot. The problem is that, after Refmac, those residues are thrown back on the disallowed regions.

This a low resolution structure (3.35A). I am using a matrix weight which renders a RMSD bond of 0.02A. Since it is very low resolution, I thought it would be a good idea to keep all residues in the allowed regions. Is there any way to tell Refmac to keep it there?

Thank you in advance,

Mario Sanches



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